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Review on the Medicinal Potentials of Waterleaf (Talinum triangulare)

2020, Mediterranean Journal of Basic and Applied Sciences (MJBAS)

Background: Human disease management in Nigerian history provides evidence of the relationship between plants and medicine. However, research and development of medicinal plants have not advanced to the stage of impacting positively on the health system in Nigeria like other advanced countries, thus, the need for this research. Methods: Stem and roots were screened for phytochemicals. The UV-VIS profile of methanolic extract of Santaloides afzelii (S. afzelii) was taken at the wavelength of 200 nm to 1200 nm. AgNPs and crude extracts were used for Anti-microbial test on the following organisms Klebsiella pneumonia, Escherichia coli, Shigella sp., Staphylococcus aureus and Salmonella typhi. Results: Methanol extracts for both Stem and roots gave the highest yields. All the phytochemicals that were present in at least one or more solvent extract in the stem and root extracts, alkaloids and steroids were present in all the solvent extract of the stem bark, while coumarin was present in all the solvent extract of the roots. Inhibition of the tested bacteria occurred more from the synthesized nanoparticles of the extracts and their respective extracting solvents except for Klebsiella sp. The UV-VIS profile of the methanol extract showed the peaks at 220 cm-1 and 260 cm-1, with the maximum absorptions at 2.59 and 1.55, respectively. Conclusion: This study revealed that AgNPs of S. afzelii stem and root extracts possess medicinal properties and antibacterial activity that inhibit bacterial growth.

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SSR Institute of International Journal of Life Science

Yilni E . Bioltif

World journal of advance research and review

ali abba gana benisheikh

Crude leave extracts of four folklore medicinal plants were subjected to phytochemical screening and antimicrobial assays against microbial pathogens using well diffusion method. The preliminary phytochemical investigation of the crude leave extracts of four folklore consists of Neem, Moringa, Jatropha and Balanites revealed that there is present of bioactive phytocomponents with potential antimicrobial ingredients when Soxhlet extraction was performed using different solvents (Hexane, Chloroform, Methanol, acetone and Ethyl acetate). The crude extracts showed significant antimicrobial activities against all microbial pathogens screened with highest activity in methanol and chloroform extracts of alkaloids as phytocomponents. While highest activity was recorded in methanol and chloroform extracts, faintly in ethyl acetate extracts using phenolics Phytochemical. Whereas, microbial activities was moderately present in chloroform, acetone and ethyl acetate extracts using steroids and reducing sugars phytocomponents respectively. Whereas, the antimicrobial activities against pathogens revealed remarkable sensitivity with prominent zone of inhibitions with ranging from 14mm to 26mm against Pseudomonas aeruginosa and 10mm to 24mm in streptococcus species using extracts from chloroform, ethyl acetate, Hexane and methanol extracts. Likewise, moderate zone of inhibition ranging from 14mm to 17mm was recorded in Staphalococcus aureus, 10mm to 17mm was recorded in P. pyogene and 10mm to 16mm in Escherichia coli respectively. Whereas weak zone of inhibition ranging from 10 mm to 12 mm was obtained against streptococcus mutans. The bioactive ingredients of those with antimicrobial activities are screened and recommended that more research work be conducted to explore their bioactive components for formulation into appropriate dosage as potential antibiotics for the treatment of infectious diseases in the study area.

Adel Al-Marby

IJSRP Journal

Twelve preparation of the ethanol and water extracts of four medicinal plants,Anogeissus leiocarpus(root and bark), Terminalia glaucescens(root and bark), Adansonia digitata(bark) and Lennea welwitschii(bark) were screened for their inhibitory effect on Escherichia coli, Salmonella typhii, Staphylococcus aureus and Shigella dysentriae using the agar-diffusion method. The efficacy of the extracts was assessed by measuring the diameter of zone of inhibition around the colonies on nutrient agar medium. The result obtained showed that the ethanol extracts (root) from T. glaucenscens and A. leiocarpus were active against all the test bacteria. The ethanol and aqueous extracts of A. digitata did not exert any inhibitory effect on test organisms, while the other eight extracts exhibited variable antibacterial activities.

Thesium viride Hill (Santalaceae) is a sub-shrub hemiparasite that grows up to 45 cm tall and widely distributed in Europe, Asia and Africa. It is used ethno medicinally in ulcer, jaundice and splenomegally. Phytochemical screening was carried out on the aqueous ethanol extract of the whole plant by using standard phytochemical methods. Antibacterial studies were also carried out on the aqueous ethanol extract by using diffusion method and broth dilution methods. The aqueous ethanol extract of the plant was found to contain alkaloids, flavonoids, anthraquinones, saponins and cardiac glycosides. The aqueous extract was found to be active against Staphylococcus aureus, Streptococcus faecalis, Escherichia coli, Helicobacter pylori and Shigella dysenteriae and had minimum inhibitory concentrations (MIC) of 10, 5, 5, 5, and 5 mg/ml and also minimum bactericidal concentration (MBC) of 20, 20, 20, 10, and 10 mg/ml respectively. The extract was also found to be inactive against Corynbacterium ulcerans and Salmonella typhii. Zones of inhibition produced by aqueous ethanol extract of plant were less than those produced by Original Research Article Shehu et al.; BJPR, 11(2): 1-6, 2016; Article no.BJPR.23046 2 ciprofloxacin (5 µg/ml). The aqueous ethanol extract of the plant could serve as an antibacterial agent on the sensitive bacteria based on the study.

Dr. Nilesh B Jawalkar

Antimicrobial activity of five solvent extracts of local plants was evaluated in vitro, with four strains of bacteria viz., Salmonella typhi, Escherichia coli, Shigella flexneri (gram-ve), Staphylococcus aureus (gram +ve) and four strains of fungi viz., Alternaria alternata, Penicillium notatum, Aspergillus niger, Penicillium digitatum microorganism. The in vitro antibacterial and antifungal activities were tested by agar disc diffusion method. The most active antibacterial plants were Vitex negundo, Tagetes erecta and antifungal plants were Xanthium strumarium, Vitex negundo and Tagetes erecta, respectively. The significant antimicrobial activities of potent extracts were compared with the standard antimicrobiotics, Ciprofloxacin and Fluconazole for bacteria as well as fungi respectively at 1 mg/ml concentration. Preliminary phytochemical analysis of V. negundo leaf, X. strumarium, M. pruriens, C. bonduc seed and T. erecta flower extracts generally revealed the presence of Alkaloids, Steroids, Terpenoids, Phenols, Saponins, Anthraquinones, Amino acids, Carotenoids, Flavonoids and Tannins at various concentrations. The results obtained in this study suggest that X. strumarium, V. negundo, T. erecta can be used in treating diseases caused by these test organisms.

International Journal of Current Micrbiology and Applied Sciences

Adetoyosi O . Daniels-Abayomi

Five local medicinal plants used in folkloric treatment of skin infection were used against four bacteria and fungi that are not principal agents in skin diseases to test for their broad spectrum antimicrobial activities. These organisms are implicated in other diseases like wound infections, urinary tract infections, pulmonary infections, zygomycosis, mucomycosis, thyphoid, shigellosis, diarrhea, respiratory tract infections e.t.c. The plants include; Butyrosporum paradoxum Pseudocedrella kotschii, Cleorodendrum capitalum, Cassia occidentalis and Piliostigma reticulatum. Solvents such as ethanol, methanol, chloroform, ethyl acetate, Nhexane and sterile water were used to extract the active components of the plants. The ethanol extracts of B. paradoxum at concentrations of between 100mg/ml to 10mg/ml were active against salmonella typhorium and Klebsiella with zones of inhibition ranging from 24mm +0.94 to 10mm + 1.19. Ethanolic extract of Pseudocedralla kotschyii was active against Salmonella typhimorium, Klebsiella and pseudomonas aeruginosa with zones of inhibition ranging from 26mm + 1.19 to 12mm + 0.84. Ethanol extract of Cleorodendrum capitalum was active against Pseudomonas aeruginosa, Salmonella typhorium and shigella with zones of inhibition ranging from 22mm + 0.89 to 12mm + 0.94. The methanolic extract of this plant was also active against P. aeruginosa and S. typhorium with inhibition range of 22mm + 1.19 and 12mm + 0.89.Ethanolic extract of P reticulatum was also active against S. typhorium and Shigella with Inhibition zones of 18mm + 1.12 and 14mm + 0.89 at relatively high concentration of between 100mg/ml and 60mm/ml. The antifungal analyses also showed activity with B. paradoxum showing fungal inhibition with zones ranging from 20mm+ 0.89 to 10mm+ 1.19 against Sycephalastrum racemosum, Synnematum spp and Giberella saffulta. The ethanolic extract of P. kotchyii showed activity against S. racemoum and S. spp with inhibition zones of between 24mm+ 0.89 to 12mm + 0.84. Ethanol extract of C. occidentalis was active against all fungal strains including Cumminghamella elegans with zones ranging from 22mm + 1.19 to 14mm+ 0.89. The methanol extract of C. capitalum was active against C. elegans with zones ranging from 20mm+ 0.89 to 12mm +1.12 while the ethanolic extract was active against G. saffulta with zones ranging from 18mm +1.19 to 12mm. + 0.84. They ethanolic extract of P. reticulatum showed static activity against S. racemosum but cidal activity against C. elegans with zones of inhibition raging from 20mm + 0.84 to 14mm+ 0.89.Phytochemical screening showed the presence of saponin, tannin, alkaloid, cardiac glycoside, phlombatannin, flavonoids e.t.c. Negative and positive control using standard antibiotics showed most of the test organisms are resistant to the antibiotics. Pseudomonas aeruginosa was sensitive to Nitrofnrantoin and gentamycin. S. racemosum was sensitive to ciprofloxacin, pefloxacin and ofloxacin G. saffulta was sensitive to ofloxacin.

International Journal of Herbal Medicine

Ashmita Pahari

International Journal of Pharmaceutical & Biological Archives

Christy Jeyaseelan

African Journal of Microbiology Research

Fatemeh Fathiazad

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Effects of Foliar Fertilization: a Review of Current Status and Future Perspectives

• Published: 09 October 2020
• Volume 21 , pages 104–118, ( 2021 )

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• Junhao Niu 1 ,
• Chang Liu 1 ,
• Mingli Huang 1 ,
• Kezhong Liu 1 &
• Dongyun Yan 1  

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The use of large amounts of chemical fertilizers promotes high-yield agriculture, but is also associated with a number of problems, such as low fertilizer utilization rates, soil acidification, and soil salinization. Comprehensive studies have shown that spraying chelated fertilizer on leaves can reduce the total amounts of fertilizer applied and achieve high fertilizer efficiency. Foliar fertilizer application after soil fertilization is an effective method to increase the contents of trace elements in crops and crop yield, and to improve the soil environment. However, the application of inorganic foliar fertilizer results in difficulties in nutrient absorption and migration in plants. Chelated foliar fertilizers are effective for improving element utilization efficiency, crop yield, and quality. The physicochemical properties, molecular structure, chelating strength, and chelating rate of chelating agents modulate the effects of application of nutrients. This study reviews and discusses the effects and problems associated with sugar alcohol–containing chelated fertilizers and foliar fertilizers.

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Abou-El-Nour E-ZAE-MA, El-Fouly MM, Salama ZAE-R (2017) Chelated Fe and Zn foliar spray improve the tolerance of kidney bean ( var. nebraska ) plants in salinized media. Biosci Res 14:525–531

Google Scholar  

Ahmad R, Waraich EA, Ashraf MY, Ahmad S, Aziz T (2014) Does nitrogen fertilization enhance drought tolerence in sunflower? A review. J Plant Nutr 37:942–963. https://doi.org/10.1080/01904167.2013.868480

Article   CAS   Google Scholar  

Alvarez RCF, Prado RM, Souza Junior JP, Oliveira RLL, Felisberto G, Deus ACF, Cruz FJR (2019) Effects of foliar spraying with new zinc sources on rice seed enrichment, nutrition, and productivity. Acta Agr Scand, Sect B 69:511–515. https://doi.org/10.1080/09064710.2019.1612939

Álvarez-Fernández A, García-Laviña P, Fidalgo C, Abadía J, Abadía A (2004) Foliar fertilization to control iron chlorosis in pear ( Pyrus communis L. ) trees. Plant Soil 263:5–15. https://doi.org/10.1023/B:PLSO.0000047717.97167.d4

Article   Google Scholar  

Amiri ME, Fallahi E, Golchin A (2008) Influence of foliar and ground fertilization on yield, fruit quality, and soil, leaf, and fruit mineral nutrients in apple. J Plant Nutr 31:515–525. https://doi.org/10.1080/01904160801895035

Artyszak A (2018) Effect of silicon fertilization on crop yield quantity and quality-a literature review in Europe. Plants (Basel, Switzerland) 7. https://doi.org/10.3390/plants7030054

Bai L, Sun W, Huang M, Li L, Geng C, Liu K, Yan D (2020) Study on the methods of separation and detection of chelates. Crit Rev Anal Chem 50:78–89. https://doi.org/10.1080/10408347.2019.1573657

Article   CAS   PubMed   Google Scholar  

Bala R, Kalia A, Dhaliwal SS (2019) Evaluation of efficacy of ZnO nanoparticles as remedial zinc nanofertilizer for rice. J Soil Sci Plant Nutr 19:379–389. https://doi.org/10.1007/s42729-019-00040-z

Barben SA, Hopkins BG, Jolley VD, Webb BL, Nichols BA, Buxton EA (2011) Zinc, manganese and phosphorus interrelationships and their effects on iron and copper in chelator-buffered solution grown Russet Burbank potato. J Plant Nutr 34:1144–1163. https://doi.org/10.1080/01904167.2011.558158

Benedicto A, Hernandez-Apaolaza L, Rivas I, Lucena JJ (2011) Determination of 67Zn distribution in navy bean ( Phaseolus vulgaris L. ) after foliar application of 67Zn-lignosulfonates using isotope pattern deconvolution. J Agric Food Chem 59:8829–8838. https://doi.org/10.1021/jf2002574

Bindraban PS, Dimkpa C, Nagarajan L, Roy A, Rabbinge R (2015) Revisiting fertilisers and fertilisation strategies for improved nutrient uptake by plants. Biol Fertil Soils 51:897–911. https://doi.org/10.1007/s00374-015-1039-7

Brown PH, Hu HN (1996) Phloem mobility of boron is species dependent: evidence for phloem mobility in sorbitol-rich species. Ann Bot (London) 77:497–506. https://doi.org/10.1006/anbo.1996.0060

Brown PH, Bellaloui N, Hu H, Dandekar A (1999) Transgenically enhanced sorbitol synthesis facilitates phloem boron transport and increases tolerance of tobacco to boron deficiency. Plant Physiol 119:17–20. https://doi.org/10.1104/pp.119.1.17

Article   CAS   PubMed   PubMed Central   Google Scholar  

Bukovac MJ, Wittwer SH (1957) Absorption and mobility of foliar. Applied Nutrients Plant Physiol 32:428–435. https://doi.org/10.1104/pp.32.5.428

Cai Z (2019) Scientifc and technological issues of nutrient management under greenhouse cultivation in China. Acta Pedofil Sin 56:1–9 (in Chinese). https://doi.org/10.11766/trxb201805310286

Cakmak I, Kalayci M, Kaya Y, Torun AA, Aydin N, Wang Y, Arisoy Z, Erdem H, Yazici A, Gokmen O, Ozturk L, Horst WJ (2010) Biofortification and localization of zinc in wheat grain. J Agric Food Chem 58:9092–9102. https://doi.org/10.1021/jf101197h

Camen D et al (2017) Changes of physiological parameters in tomatoes under salt stress and fertilization levels. Rom Biotech Let 22:12821–12826

CAS   Google Scholar  

Chakraborty B, Singh PN, Kumar S, Srivastava PC (2014a) Uptake and distribution of iron from different iron sources applied as foliar sprays to chlorotic leaves of low-chill peach cultivars. Agric Res 3:293–301. https://doi.org/10.1007/s40003-014-0128-4

Chakraborty B, Singh PN, Singh AK, Srivastava PC (2014b) Evaluation of different iron sources for iron chlorosis recovery in low-chill peach cultivars. J Plant Nutr 37:224–231. https://doi.org/10.1080/01904167.2013.859693

Cheng L, Zhang B, Wang J, Shi Y, Chen K (2011) Research progress of humic-acid containing fertilizer. Chin Soils Fert 5:1–6 (in Chinese). https://doi.org/10.3969/j.issn.1673-6257.2011.05.001

Davarpanah S, Tehranifar A, Davarynejad G, Aran M, Abadía J, Khorassani R (2017) Effects of foliar nano-nitrogen and urea fertilizers on the physical and chemical properties of pomegranate ( Punica granatum cv. Ardestani ) fruits. Hortscience. 52:288–294. https://doi.org/10.21273/hortsci11248-16

Davarpanah S, Tehranifar A, Zarei M, Aran M, Davarynejad G, Abadía J (2020) Early season foliar iron fertilization increases. Fruit Yield Qual Pomegranat Agron 10:832. https://doi.org/10.3390/agronomy10060832

del Amor FM, Cuadra-Crespo P, Varo P, Gomez MC (2009) Influence of foliar urea on the antioxidant response and fruit color of sweet pepper under limited N supply. J Sci Food Agric 89:504–510. https://doi.org/10.1002/jsfa.3485

Ding SS et al (2015) Effects of small molecular organics chelated calcium fertilizer on cherry tomato yield,quality and nutrients absorption. Chin Soils Fert 61–66 (in Chinese). https://doi.org/10.11838/sfsc.20150511

Dong ZQ, Zhang LH, Pei CJ, Zhao SJ, Jia XL, Zhang MC (2011) Study on technique of relieving lack iron nutrition for soybean plants in calcareous soil. Acta Agric Boreali-Sinica 26:122–124 (in Chinese)

Doolette C, Read T, Li C, Scheckel K, Donner E, Schjoerring J, Lombi E (2018) Foliar application of zinc sulphate and zinc EDTA to wheat leaves: differences in mobility, distribution, and speciation. J Exp Bot 69:4469–4481. https://doi.org/10.1093/jxb/ery236

Du Y et al (2015) In situ analysis of foliar zinc absorption and short-distance movement in fresh and hydrated leaves of tomato and citrus using synchrotron-based X-ray fluorescence microscopy. Ann Bot 115:41–53. https://doi.org/10.1093/aob/mcu212

Du W, Pan Z-Y, Hussain SB, Han Z-X, Peng S-A, Liu Y-Z (2020) Foliar supplied boron can be transported to roots as a boron-sucrose complex via phloem in citrus trees. Front Plant Sci 11:250 (in Chinese). https://doi.org/10.3389/fpls.2020.00250

Article   PubMed   PubMed Central   Google Scholar  

Fan J, Zheng SZ, Hu HQ, Huang ZL, Fu QL, Huang ZL (2010) Effect of spraying foliar fertilizer on pakchoi with the treatment of different base fertilizers. Chin Soils Fert 28:25–30 (in Chinese). https://doi.org/10.3724/SP.J.1011.2010.01385

Fernández V, Brown PH (2013) From plant surface to plant metabolism: the uncertain fate of foliar-applied nutrients. Front Plant Sci 4:289. https://doi.org/10.3389/fpls.2013.00289

Fernández V, Eichert T (2009) Uptake of hydrophilic solutes through plant leaves: current state of knowledge and perspectives of foliar fertilization. Crit Rev Plant Sci 28:36–68. https://doi.org/10.1080/07352680902743069

Fernández V, Ebert G, Winkelmann G (2005) The use of microbial siderophores for foliar iron application studies. Plant Soil 272:245–252. https://doi.org/10.1007/s11104-004-5212-2

Fernández V, Sotiropoulos T, Brown PH (2013) Foliar fertilization: scientific principles and field pratices. International fertilizer industry association Paris, France

Gao SD, Yang CY, Deng XP, Xia Y, Shen ZG, Chen YH (2018) Study on absorption and transport of K and Zn by foliar application in tobacco leaves. J Nanjing Agric Univ 41:330–340 (in Chinese). https://doi.org/10.7685/jnau.201706033

Ghasemi S, Khoshgoftarmanesh AH, Afyuni M, Hadadzadeh H (2013) The effectiveness of foliar applications of synthesized zinc-amino acid chelates in comparison with zinc sulfate to increase yield and grain nutritional quality of wheat. Eur J Agron 45:68–74. https://doi.org/10.1016/j.eja.2012.10.012

Gong XT (2002) Chelated multi–compound micronutrient fertilizer. Plant Nutr Fert Sci 8:127 (in Chinese). https://doi.org/10.3321/j.issn:1008-505X.2002.01.026

Guan X, Yang Y, Wang H, Guo C, Guan J (2014) Effects of spraying calcium on contents of calcium and pectin and fruit quality of Red Globe Grape ( Vitis vinifera L. ). Plant Nutr Fert Sci 20:179–185 (in Chinese). https://doi.org/10.11674/zwyf.2014.0120

Guo DY, Xie JL, Zhu SG, Liu HX, Miao YF, Qiao Q (2008) Effects of foliar application of Zn fertilizer on nitrate content and nitrate reductase (NR) activity in different lettuce organs. Acta Agric Boreali-Occident Sin 17:302–305 (in Chinese). https://doi.org/10.3969/j.issn.1004-1389.2008.05.066

Hamze MR, Khoshgoftarmanesh AH, Shariatmadari H, Baninasab B (2018) The effects of foliar applied potassium in the mineral form and complexed with amino acids on pistachio nut yield and quality. Arch Agron Soil Sci 64:1432–1445. https://doi.org/10.1080/03650340.2018.1439580

Han DF, Wang DH, Huang PZ, Duan JX, Ge RS (2011) Effects of different combined manganese ion on the yield and quality of Brassica pekinensis. J South China Agric Univ 32:10–13 (in Chinese). https://doi.org/10.3969/j.issn.1001-411X.2011.04.003

Hassan IA, Zeid HM, Taia W, Haiba NS, Zahran A, Badr RH, Dakak RA, Shalaby EA (2015) Fertilization regimes under hot conditions alter photosynthetic response of bean plants. Photosynthetica. 53:157–160. https://doi.org/10.1007/s11099-015-0090-9

He X, Karim A, Mahemuti K (2012) Correlative study of foliage fertilization on Korla fragrant pears for improving cold resistance. Xinjiang Agric Sci 49:1401–1407 (in Chinese)

He W, Shohag MJ, Wei Y, Feng Y, Yang X (2013) Iron concentration, bioavailability, and nutritional quality of polished rice affected by different forms of foliar iron fertilizer. Food Chem 141:4122–4126. https://doi.org/10.1016/j.foodchem.2013.07.005

He J, Nie ZG, Li LY, Huang ML, Zhang FK, Xu RC, Yan DY (2017) The application effects of chelate fertilizers in agriculture. Chin J Soil Sci 48:507–512 (in Chinese). https://doi.org/10.19336/j.cnki.trtb.2017.02.37

He J, Huang M, Li L, Nie Z, Geng C, Yan D (2019a) Stability and structural characterization of chelated fertilizers. Spectrosc Spectr Anal 39:2966–2973

He J, Zhang F, Lu Y, Zhu K, He J, Li P, Yan D (2019b) Reation conditions of sugar alcohol chelated calcium fertilizer and their influence on chelated rate. Environ Eng 37:160–164 (in Chinese)

Hosseini SA, Rethore E, Pluchon S, Ali N, Billiot B, Yvin J-C (2019) Calcium application enhances drought stress tolerance in sugar beet and promotes plant biomass and beetroot sucrose concentration Int. J Mol Sci 20. https://doi.org/10.3390/ijms20153777

Hu Y, Burucs Z, Schmidhalter U (2008) Effect of foliar fertilization application on the growth and mineral nutrient content of maize seedlings under drought and salinity. Soil Sci Plant Nutr 54:133–141. https://doi.org/10.1111/j.1747-0765.2007.00224.x

Januškaitienė I, Kacienė G (2017) The effect of foliar spray fertilizers on the tolerance of Hordeum vulgare to UV-B radiation and drought stress. Cereal Res Commun 45:390–400. https://doi.org/10.1556/0806.45.2017.030

Khoshgoftarmanesh AH, Schulin R, Chaney RL, Daneshbakhsh B, Afyuni M (2010) Micronutrient-efficient genotypes for crop yield and nutritional quality in sustainable agriculture. A review Agron Sustain Dev 30:83–107. https://doi.org/10.1051/agro/2009017

Kumar O, Singh SK, Latare AM, Yadav SN (2018) Foliar fertilization of nickel affects growth, yield component and micronutrient status of barley ( Hordeum vulgare L .) grown on low nickel soil. Arch Agron Soil Sci 64:1407–1418. https://doi.org/10.1080/03650340.2018.1438600

Lester GE, Grusak MA (2004) Field application of chelated calcium: postharvest effects on cantaloupe and honeydew fruit quality. Horttechnology 14:29–38. https://doi.org/10.21273/horttech.14.1.0029

Li M, Liu Z (2015) Effects of reducing application amount of base fertilizer and increasing application time of leaf fertilizer on corn yield. Agric Sci Technol 16:947

Li YT, Li XY, Xiao Y, Zhao BQ, Wang LX (2009) Advances in study on mechanism of foliar nutrition and development of foliar fertilizer application. Sci Agric Sin 42:162–172 (in Chinese). https://doi.org/10.3864/j.issn.0578-1752.2009.01.020

Li J, Chen D, Sun S, Xie H, Tu M, Jiang G (2014a) Effects of foliage application of calcium on fruit quality related indexes and split-pit of peach. J Southwest-China Agric Sci 27:896–898 (in Chinese)

Li YQ, Li PC, Liu AZ, Dong HL (2014b) Research progress on foliar fertilizer of cotton. Chin Agric Sci Bull 30:15–19 (in Chinese)

Li WZ, Li WH, Xu MY, Wang L (2018) Analysis of mineral elements in maize kernel treated with leaf fertilizers. Biotechnol Bull 34:110–116 (in Chinese). https://doi.org/10.13560/j.cnki.biotech.bull.1985.2017-0957

Li P, Geng C, Li L, Li Y, Li T, Wei Q, Yan D (2020a) Calcium-sorbitol chelating technology and application in potatoes. Am J Biochem Biotechnol 16:96–102. https://doi.org/10.3844/ajbbsp.2020.96.102

Li Y, Yang G, Li F, Bai L, Huang M, Liu K, Yan D (2020b) Effects of sugar alcohol chelated calcium fertilizer on yield, quality and nutrient uptake of potato. Soil 52:773–780 (in Chinese). https://doi.org/10.13758/j.cnki.tr.2020.04.017

Litalien A, Zeeb B (2020) Curing the earth: a review of anthropogenic soil salinization and plant-based strategies for sustainable mitigation. Sci Total Environ 698. https://doi.org/10.1016/j.scitotenv.2019.134235

Lötze E, Hoffman EW (2014) Foliar application of calcium plus boron reduces the incidence of sunburn in ‘Golden Delicious’ apple. J Hortic Sci Biotechnol 89:607–612. https://doi.org/10.1080/14620316.2014.11513127

Lucena JJ (2003) Fe chelates for remediation of Fe chlorosis in strategy I plants. J Plant Nutr 26:1969–1984. https://doi.org/10.1081/PLN-120024257

Luo Z, Kong X, Dai J, Dong H (2015) Soil plus foliar nitrogen application increases cotton growth and salinity tolerance. J Plant Nutr 38:443–455. https://doi.org/10.1080/01904167.2014.912324

Machado RMA, Serralheiro RP (2017) Soil salinity: effect on vegetable crop growth. Management practices to prevent and mitigate soil salinization. Horticulturae 3:13. https://doi.org/10.3390/horticulturae3020030

Manzi M, Lado J (2019) Foliar applications of calcium do not impact on fruit and leaf nutrient concentration or quality of ‘O’Neal’ blueberry. J Hortic Sci Biotechnol 94:1–9. https://doi.org/10.1080/14620316.2019.1577185

Marešová J, Remenárová L, Horník M, Pipíška M, Augustín J, Lesný J (2012) Foliar uptake of zinc by vascular plants: radiometric study. J Radioanal Nucl Chem 292:1329–1337. https://doi.org/10.1007/s10967-012-1642-0

Martínez-Ballesta MC, Dominguez-Perles R, Moreno DA, Muries B, Alcaraz-López C, Bastías E, García-Viguera C, Carvajal M (2010) Minerals in plant food: effect of agricultural practices and role in human health. A review. Agron Sustain Dev 30:295–309. https://doi.org/10.1051/agro/2009022

Modaihsh AS (1997) Foliar application of chelated and non-chelated metals for supplying micronutrients to wheat grown on calcareous soil. Exp Agric 33:237–245. https://doi.org/10.1017/S001447979700001X

Montalvo D, Degryse F, Da Silva RC, Baird R, McLaughlin MJ (2016) Agronomic effectiveness of zinc sources as micronutrient fertilizer. Adv Agron 139:215–267. https://doi.org/10.1016/bs.agron.2016.05.004

Moon JY et al (2019) Influence of foliar fertilization with monopotassium phosphate on growth and yield of sweet potato ( Ipomoea batatas L. ). Korean J Soil Sci Fert 52:217–225 (in Korean)

Mu J, Xu YC, Zhu PM, Raza W, Shen QR (2006) Response of rice cultivated in aerobic soil to amino acid chelated micronutrient fertilizer. J Soil Water Conserv 178-182 (in Chinese). https://doi.org/10.3321/j.issn:1009-2242.2006.05.044

Muller da Silva PH, Campoe OC, Vieira IC, de Paula RC (2015) Leaf boron application in eucalypt under water stress. Sci For 43:395–405

Naeem M, Naeem MS, Ahmad R, Ihsan MZ, Ashraf MY, Hussain Y, Fahad S (2018) Foliar calcium spray confers drought stress tolerance in maize via modulation of plant growth, water relations, proline content and hydrogen peroxide activity. Arch Agron Soil Sci 64:116–131. https://doi.org/10.1080/03650340.2017.1327713

Neuhaus C, Geilfus CM, Mühling KH (2014) Increasing root and leaf growth and yield in Mg-deficient faba beans ( Vicia faba ) by MgSO 4 foliar fertilization. J Plant Nutr Soil Sci 177:741–747. https://doi.org/10.1002/jpln.201300127

Oliveira KS, de Mello PR, de Farias Guedes VH (2020) Leaf spraying of manganese with silicon addition is agronomically viable for corn and Sorghum plants. J Soil Sci Plant Nutr 20:872–880. https://doi.org/10.1007/s42729-020-00173-6

Otálora G, Piñero MC, López-Marín J, Varó P, del Amor FM (2018) Effects of foliar nitrogen fertilization on the phenolic, mineral, and amino acid composition of escarole ( Cichorium endivia L. var. latifolium ). Sci Hortic-Amsterdam 239:87–92. https://doi.org/10.1016/j.scienta.2018.05.031

Papadakis IE, Protopapadakis E, Therios IN, Tsirakoglou V (2005) Foliar treatment of Mn deficient ‘Washington navel’orange trees with two Mn sources. Sci Hortic-Amsterdam 106:70–75. https://doi.org/10.1016/j.scienta.2005.02.015

Pei J, Li Y, Cheng C, Li Z (2018) Effects of different calcium agents on fruit firmness and related cell wall metabolites in Hanfu’apple. J Fruit Sci 35:1059–1066 (in Chinese). https://doi.org/10.13925/j.cnki.gsxb.20180169

Peryea FJ (2006) Phytoavailability of zinc in postbloom zinc sprays applied to ‘Golden Delicious’ apple trees. Horttechnology. 16:60–65. https://doi.org/10.21273/HORTTECH.16.1.0060

Phattarakul N et al (2012) Biofortification of rice grain with zinc through zinc fertilization in different countries. Plant Soil 361:131–141. https://doi.org/10.1007/s11104-012-1211-x

Poblaciones MJ, Rengel Z (2016) Soil and foliar zinc biofortification in field pea ( Pisum sativum L. ): grain accumulation and bioavailability in raw and cooked grains. Food Chem 212:427–433. https://doi.org/10.1016/j.foodchem.2016.05.189

Pourjafar L, Zahedi H, Sharghi Y (2016) Effect of foliar application of nano iron and manganese chelated on yield and yield component of canola ( Brassica napus L. ) under water deficit stress at different plant growth stages. Agric Sci Dig 36:172–178

Qi HY, Wang D, Qi MF, Liu YF, He Y, Li TL (2014) Regulation of different calcium forms on the photosynthesis of tomato leaves under heat stress. Chin J Appl Ecol 25:3540–3546

Rady MM, El-Shewy AA, Seif El-Yazal MA, Abd El-Gawwad IFM (2019) Integrative application of soil P-solubilizing bacteria and foliar nano P improves phaseolus vulgaris plant performance and antioxidative defense system components under calcareous soil conditions. J Soil Sci Plant Nutr 20:820–839. https://doi.org/10.1007/s42729-019-00168-y

Raliya R, Nair R, Chavalmane S, Wang W-N, Biswas P (2015) Mechanistic evaluation of translocation and physiological impact of titanium dioxide and zinc oxide nanoparticles on the tomato ( Solanum lycopersicum L. ) plant. Metallomics. 7:1584–1594. https://doi.org/10.1039/C5MT00168D

Raliya R, Franke C, Chavalmane S, Nair R, Reed N, Biswas P (2016) Quantitative understanding of nanoparticle uptake in watermelon. Plants Front Plant Sci 7:1288–1288. https://doi.org/10.3389/fpls.2016.01288

Article   PubMed   Google Scholar  

Raliya R, Saharan V, Dimkpa C, Biswas P (2018) Nanofertilizer for precision and sustainable agriculture: current state and future perspectives. J Agric Food Chem 66:6487–6503. https://doi.org/10.1021/acs.jafc.7b02178

Reed DW, Lyons CG Jr, McEachern GR (1988) Field evaluation of inorganic and chelated iron fertilizers as foliar sprays and soil application. J Plant Nutr 11:1369–1378. https://doi.org/10.1080/01904168809363894

Rios JJ, Carrasco-Gil S, Abadía A, Abadía J (2016) Using Perls staining to trace the iron uptake pathway in leaves of a Prunus rootstock treated with iron foliar fertilizers. Front Plant Sci 7:893. https://doi.org/10.3389/fpls.2016.00893

Rodríguez-Lucena P, Tomasi N, Pinton R, Hernández-Apaolaza L, Lucena JJ, Cesco S (2009) Evaluation of 59 Fe-lignosulfonates complexes as Fe-sources for plants. Plant Soil 325:53–63. https://doi.org/10.1007/s11104-009-0091-1

Rodríguez-Lucena P, Hernández-Apaolaza L, Lucena JJ (2010) Comparison of iron chelates and complexes supplied as foliar sprays and in nutrient solution to correct iron chlorosis of soybean. J Plant Nutr Soil Sci 173:120–126. https://doi.org/10.1002/jpln.200800256

Rodríguez-Lucena P, Benedicto A, Lucena JJ, Rodríguez-Castrillón JA, Moldovan M, García Alonso JI, Hernández-Apaolaza L (2011) Use of the stable isotope 57Fe to track the efficacy of the foliar application of lignosulfonate/Fe3+ complexes to correct Fe deficiencies in cucumber plants. J Sci Food Agric 91:395–404. https://doi.org/10.1002/jsfa.4197

Roosta HR (2014) Effects of foliar spray of K on mint, radish, parsley and coriander plants in aquaponic system. J Plant Nutr 37:2236–2254. https://doi.org/10.1080/01904167.2014.920385

Roosta HR, Hamidpour M (2013) Mineral nutrient content of tomato plants in aquaponic and hydroponic systems: effect of foliar application of some macro-and micro-nutrients. J Plant Nutr 36:2070–2083. https://doi.org/10.1080/01904167.2013.821707

Roosta HR, Mohsenian Y (2012) Effects of foliar spray of different Fe sources on pepper ( Capsicum annum L. ) plants in aquaponic system. Sci Hortic-Amsterdam 146:182–191. https://doi.org/10.1016/j.scienta.2012.08.018

Rossi L, Fedenia L, Sharifan H, Ma X, Lombardini L (2018) Effects of foliar application of zinc sulfate and zinc nanoparticles in coffee ( Coffea arabica L. ) plants. Plant Physiol Biochem 135. https://doi.org/10.1016/j.plaphy.2018.12.005

Schiavon M, Dall’acqua S, Mietto A, Pilon-Smits EA, Sambo P, Masi A, Malagoli M (2013) Selenium fertilization alters the chemical composition and antioxidant constituents of tomato ( Solanum lycopersicon L. ). J Agric Food Chem 61:10542–10554. https://doi.org/10.1021/jf4031822

Schlegel TK, Schonherr J, Schreiber L (2006) Rates of foliar penetration of chelated Fe(III): role of light, stomata, species, and leaf age. J Agric Food Chem 54:6809–6813. https://doi.org/10.1021/jf061149i

Schönherr J, Fernández V, Schreiber L (2005) Rates of cuticular penetration of chelated FeIII: role of humidity, concentration, adjuvants, temperature, and type of chelate. J Agric Food Chem 53:4484–4492. https://doi.org/10.1021/jf050453t

Shahid M, Saleem MF, Saleem A, Sarwar M, Khan HZ, Shakoor A (2020) Foliar potassium-induced regulations in glycine betaine and malondialdehyde were associated with grain yield of heat-stressed bread wheat ( Triticum aestivum L. ). J Soil Sci Plant Nutr. https://doi.org/10.1007/s42729-020-00250-w

Shahverdi MA, Omidi H, Tabatabaei SJ (2018) Plant growth and steviol glycosides as affected by foliar application of selenium, boron, and iron under NaCl stress in Stevia rebaudiana. Bertoni Ind Crop Prod 125:408–415. https://doi.org/10.1016/j.indcrop.2018.09.029

Shahverdi MA, Omidi H, Damalas CA (2020) Foliar fertilization with micronutrients improves Stevia rebaudiana tolerance to salinity stress by improving root characteristics. Braz J Bot 43:55–65. https://doi.org/10.1007/s40415-020-00588-6

Shalaby T, Bayoumi Y, Alshaal T, Elhawat N, Sztrik A, El-Ramady H (2017) Selenium fortification induces growth, antioxidant activity, yield and nutritional quality of lettuce in salt-affected soil using foliar and soil applications. Plant Soil 421:245–258. https://doi.org/10.1007/s11104-017-3458-8

Shams K (2019) Alleviation of terminal drought stress in wheat by foliar application of zinc and manganese. Soil Environ (Faisalabad, Pak) 38:103–111. https://doi.org/10.25252/se/19/71650

Shen X et al (2016) Application of small molecular organics chelated calcium fertilizer. Chin Soils Fert:87–92 (in Chinese). https://doi.org/10.11838/sfsc.20160314

Shen X, Li YT, Yuan L, Zhao BQ, Lin ZA, Yang XD, Li J (2017) Effects of combined foliar application of amino acids with Zn increase the biomass, quality and zinc use efficiency of Chinese cabbage. Plant Nutr Fert Sci 23:181–188 (in Chinese). https://doi.org/10.11674/zwyf.16090

Souri MK, Sooraki FY, Moghadamyar M (2017) Growth and quality of cucumber, tomato, and green bean under foliar and soil applications of an aminochelate fertilizer. Hortic Environ Biotel 58:530–536. https://doi.org/10.1007/s13580-017-0349-0

Stein AJ (2010) Global impacts of human mineral malnutrition. Plant Soil 335:133–154

Sun L et al (2011) The effect of reducing fertilizer application on tomato production, quality and soil nitrate in Chaohu lake basin. Chin Agric Sci Bull 27:250–255 (in Chinese)

Sun X, Wang Y, Zhang C, Zhang J, Qiu H, Wang D, Li D (2015) Effects of different kinds of zinc fertilizers on zinc absorption, accumulation and distribution of potato under rainfed conditions. Agric Res Arid Areas 33:072–078 (in Chinese). https://doi.org/10.7606/j.issn.1000-7601.2015.03.12

Sun CW, Chen Z, Zhao YZ, Niu SK, Yang LL (2017) Absorption,distribution and utilization of 15 N in Jumeigui seedlings under different fertilization methods. Acta Agric Boreali-Sinica 32:260–264 (in Chinese). https://doi.org/10.7668/hbnxb.2017.S1.045

Teodoro PE, Ribeiro LP, de Oliveira EP, Guedes Correa CC, Torres FE (2015) Dry mass in soybean in response to application leaf with silicon under conditions of water deficit. Biosci J 31:161–170

Tucker MD, Barton LL, Thomson BM, Wagener BM, Aragon A (1999) Treatment of waste containing EDTA by chemical oxidation. Waste Manag 19:477–482. https://doi.org/10.1016/S0956-053X(99)00235-4

Vicosi KA, de Carvalho AS, Silva DC, Almeida FP, Ribeiro D, Flores RA (2020) Foliar fertilization with boron on the growth, physiology, and yield of snap beans. J Soil Sci Plant Nutr 20:1–8. https://doi.org/10.1007/s42729-020-00178-1

Wallace GA, Wallace A (1982) Micronutrient uptake by leaves from foliar sprays of EDTA chelated metals. J Plant Nutr 5:975–978. https://doi.org/10.1080/01904168209363030

Wang XK, Li HS, Liu WD, Wu SH, Shun JJ (2000) Effects of calcium chelator on the nitrogen metabolism and dry matter accumulation in wheat seedlings. Plant Nutr Fert Sci 6:42–47 (in Chinese)

Wang JW, Mao H, Zhao HB, Huang DL, Wang ZH (2012) Different increases in maize and wheat grain zinc concentrations caused by soil and foliar applications of zinc in Loess Plateau. China Field Crop Res 135:89–96. https://doi.org/10.1016/j.fcr.2012.07.010

Wang JW, Wang ZH, Mao H, Zhao HB, Huang DL (2013) Increasing Se concentration in maize grain with soil-or foliar-applied selenite on the Loess Plateau in China field. Crop Res 150:83–90. https://doi.org/10.1016/j.fcr.2013.06.010

Wang LJ, Jiang DX, Chen ZP, Wei J, Wan SQ, Ma ZW (2014) Effects of applying chelated fertilizer to nicotine and potassium in the flue-cured tobacco leaf. J South China Agric Univ 35:35–39 (in Chinese)

Wang SP, Hong YC, Huang FX (2015) Review of foliar fertilizer development status. J Anhui Agric Univ 43:96–98 (in Chinese). https://doi.org/10.3969/j.issn.0517-6611.2015.04.033

Wang LL, Gao ZS, Song WJ, Song T (2017) Effects of different boron fertilizer on eggplant growth,yield and quality. J Hubei Agric Sci 56:4275–4277 (in Chinese). https://doi.org/10.14088/j.cnki.issn0439-8114.2017.22.016

Wang Y et al (2018) Effects of foliar application of boron fertilizer on rhizosphere soil nutrient, plant growth and yield of tartary buckwheat. J South Agric 49:253–257 (in Chinese). https://doi.org/10.3969/j.issn.2095-1191.2018.02.08

Wang LQ, Li PC, Huang ML, Liu KZ, Geng CZ, Yan DY (2019) Research progress in the application of sugar alcohol in food medicine and agriculture. Sci Technol Food Ind 40:337–340+345 (in Chinese). https://doi.org/10.13386/j.issn1002-0306.2019.07.058

Wei Y, Shohag MJ, Yang X, Yibin Z (2012a) Effects of foliar iron application on iron concentration in polished rice grain and its bioavailability. J Agric Food Chem 60:11433–11439. https://doi.org/10.1021/jf3036462

Wei Y, Shohag MJI, Yang XE (2012b) Biofortification and bioavailability of rice grain zinc as affected by different forms of foliar zinc fertilization. PLoS One 7. https://doi.org/10.1371/journal.pone.0045428

Wei DP, Wei JF, Wu XK, Liu HY, Xiong JW, Shi DN (2013) Effect of foliage spray on dry matter accumulation, nutrient status and soil nutrient of potato. J Henan Agric Sci 42:57–61 (in Chinese). https://doi.org/10.3969/j.issn.1004-3268.2013.08.014

Will S, Eichert T, Fernández V, Möhring J, Müller T, Römheld V (2011) Absorption and mobility of foliar-applied boron in soybean as affected by plant boron status and application as a polyol complex. Plant Soil 344:283–293. https://doi.org/10.1007/s11104-011-0746-6

Will S, Eichert T, Fernández V, Müller T, Römheld V (2012) Boron foliar fertilization of soybean and lychee: effects of side of application and formulation adjuvants. J Plant Nutr Soil Sci 175:180–188. https://doi.org/10.1002/jpln.201100107

Wu HZ, Liu DH, Wang PY, Zhao HY (2011) Effects of different applying methods of Cu and Zn on growth and Tanshinone of Salviae. Miltiorrhizae Bunge Soils 43:781–786 (in Chinese)

Wu WQ, Liu Y, Li P, Zhao Y, Mou GX (2013) Effects of sugar alcohol complex calcium on eggplant growth, yield and quality. China Vegetables:46–48 (in Chinese). https://doi.org/10.3969/j.issn.1000-6346.2013.24.010

Xiao Y, Cao YP, Wang JG, Chen K (2004) Research of mixed adjuvants on the absorption of nutrient elements in crop leaf Plant. Nutr Fert Sci 10:281–285 (in Chinese). https://doi.org/10.3321/j.issn:1008-505X.2004.03.012

Xiao H, Rodrigues RR, Bonierbale M, Veilleux R, Williams M (2018) Foliar application of Fe resonates to the belowground rhizosphere microbiome in Andean landrace potatoes. Appl Soil Ecol 131:89–98. https://doi.org/10.1016/j.apsoil.2018.08.006

Xu FL, Liang YL, Zhang CE, Du SN, Chen ZJ (2004) Effect of fertilization on distribution of nitrate in cucumber and soil in sunlight greenhouse. Plant Nutr Fert Sci 10:68–72 (in Chinese). https://doi.org/10.3321/j.issn:1008-505X.2004.01.013

Xu N, Zhang FY, Cao N, Wang C, Liu GJ, Liu M, Sun XH (2018) The effect of silicon foliar fertilizer on the rhizosphere soil microecology in the wheat-maize system. J Anhui Agric Univ 45:363–366 (in Chinese). https://doi.org/10.13610/j.cnki.1672-352x.20180427.025

Yan L, Jiang C, Dong X, Wu X, Riaz M, Lu X (2017a) Effects of polyol-chelated boron fertilizers on physiological characteristics of rapeseed seedlings. J Huazhong Agric Univ 36:38–44 (in Chinese)

Yan L, Jiang C, Muhammad R, Wu X, Lu X, Du C, Wang Y (2017b) Mitigative effect of different forms of boron on aluminum toxicity of rape seedlings and its FTIR characteristics. Acta Agron Sin 43:1817–1826. https://doi.org/10.3724/SP.J.1006.2017.01817

Yan DY et al. (2018a): Determination of chelation rate of sugar alcohol chelated calcium fertilizer by conductivity measurement. China Patent CN109142450A

Yan DY et al. (2018b) Determination of chelation rate of sugar alcohol chelated calcium fertilizer by Spectrophotometry. China Patent CN109100312A

Yang Y, Wang YM, Wu XY, Wei YF, Yang LY, Gong L (2014) Effects of spraying calcium on photosynthesis and the activities of superoxide dismutases, peroxidase, catalase of Guifeimeigui grapevine. Sino-Overseas Grapevine Wine 23-26 (in chinese). https://doi.org/10.13414/j.cnki.zwpp.2014.04.005

Yu HY, Li TX, Zhou JM (2005) Secondary salinization of greenhouse soil and its effects on soil properties. Soils. 37:581–586 (in Chinese)

Yu HL, Lin ZA, Li YT, Yuan L, Zhao BQ (2014) Effects of spraying low molecular organic compounds on growth and nutrients uptake of rape ( Brassica Chinensis L. ). Plant Nutr Fert Sci 20:1560–1568 (in Chinese)

Yu H, Si P, Qiao X, Yang X, Gao D, Liu Z (2016) Iron absorption and quality of strawberry affected by different forms of foliar iron fertilizer. Chin Soils Fert 73–78 (in Chinese).  https://doi.org/10.11838/sfsc.20160513

Yu H, Peng S, Wei S, Qiao X, Gao D, Yang X, Wang Z (2017) Effects of spraying calcium fertilizer on calcium content and quality of peach. Chin Agric Sci Bull 33:63–67 (in Chinese)

Yuan ZW, VanBriesen JM (2006) The formation of intermediates in EDTA and NTA biodegradation. Environ Eng Sci 23:533–544. https://doi.org/10.1089/ees.2006.23.533

Yuan W, Dong YH, Wang H (2009) A review: biological effect of plant amino acids trace-element fertilizers. Soils. 41:16–20 (in Chinese)

Yuan L, Wu L, Yang C, Lv Q (2013) Effects of iron and zinc foliar applications on rice plants and their grain accumulation and grain nutritional quality. J Sci Food Agric 93:254–261. https://doi.org/10.1002/jsfa.5749

Yunta F, Martín I, Lucena JJ, Gárate A (2013) Iron chelates supplied foliarly improve the iron translocation rate in Tempranillo grapevine Commun. Soil Sci Plan 44:794–804. https://doi.org/10.1080/00103624.2013.748862

Zafar S, Ashraf MY, Anwar S, Ali Q, Noman A (2016) Yield enhancement in wheat by soil and foliar fertilization of K and Zn under saline environment. Soil Environ (Faisalabad, Pak) 35:46–55

Zahedi SM, Abdelrahman M, Hosseini MS, Hoveizeh NF, Tran L-SP (2019) Alleviation of the effect of salinity on growth and yield of strawberry by foliar spray of selenium-nanoparticles. Environ Pollut (Oxford, U K) 253:246–258. https://doi.org/10.1016/j.envpol.2019.04.078

Zargar M, Tumanyan A, Ivanenko E, Dronik A, Tyutyuma N, Pakina E (2019) Impact of foliar fertilization on apple and pear trees in reconciling productivity and alleviation of environmental concerns under arid conditions. Commun Integr Biol 12:1–9. https://doi.org/10.1080/19420889.2019.1565252

Zhang CM, Zhou XB (2019) Effects of different selenium application methods on Se utilization efficiency of rice. J Acta Pedologica Sinica 56:186–194 (in Chinese). https://doi.org/10.11766/trxb201805110096

Zhang M, Hu CX, Sun XC (2011) Effects of spraying micronutrient and amino acids into surface of leaves on yield and quality of Chinese cabbage. J Huazhong Agric Univ 30:613–617 (in Chinese)

Zhang Y et al (2013) Zinc sulfate and sugar alcohol zinc sprays at critical stages to improve apple fruit quality. Horttechnology 23:490–497. https://doi.org/10.21273/horttech.23.4.490

Zhang Y, Fu C, Yan Y, Fan X, Wang Y, Li M (2014) Foliar application of sugar alcohol zinc increases sugar content in apple fruit and promotes activity of metabolic enzymes. Hortscience 49:1067–1070. https://doi.org/10.21273/hortsci.49.8.1067

Zhang C, Jia HF, Wang J, Jiu ST, Wang MQ (2016) Effectiveness and concentration of foliar-application of potassium fertilizers on grapevine evaluated by expression of potassium uptake related genes. Plant Nutr Fert Sci 22:1091–1101 (in Chinese). https://doi.org/10.11674/zwyf.15251

Zhang SX et al (2017) Effects of different trace element fertilizers on strawberry seedling growth. Soils 49:261–267 (in Chinese). https://doi.org/10.13758/j.cnki.tr.2017.02.008

Zhang L, Liu L, Wang Y, Lu B, Yao Z, Jiang C (2019) Different influences of organic and inorganic boron fertilizers on citrange rootstock growth and physiology characters. Acta Horticulturae Sinica 46:135–142 (in Chinese). https://doi.org/10.16420/j.issn.0513-353x.2018-0157

Zheng YH, Pan GY, Mao GJ, Wan B, Hu J (2007) The effect of potassium fertilizer (as the foliage dressing) on potato yield with virus-free. Guizhou Agric Sci 35:69–70 (in Chinese). https://doi.org/10.3969/j.issn.1001-3601.2007.01.024

Zheng XC, Wang HB, Wang XD, Wang BL, Shi XB, Liu FZ (2016) Researches on selenium absorption and distribution in Kyoho grape. Chin Soils Fert 128-132 (in Chinese). https://doi.org/10.11838/sfsc.20160422

Zheng CS et al (2018) Effects of foliar nitrogen applications on the absorption of nitrate nitrogen by cotton roots. Cotton Sci 30:338–343 (in Chinese). https://doi.org/10.11963/1002-7807.zcsdhl.20180703

Zhu Q, Zhang M, Ma Q (2012) Copper-based foliar fertilizer and controlled release urea improved soil chemical properties, plant growth and yield of tomato. Sci Hortic-Amsterdam 143:109–114. https://doi.org/10.1016/j.scienta.2012.06.008

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This work is supported by the National Natural Science Foundation of China (No. 31972516), and the Major Research Project of Shandong Province (Public Welfare Special) (No. 2017GNC11116).

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Niu, J., Liu, C., Huang, M. et al. Effects of Foliar Fertilization: a Review of Current Status and Future Perspectives. J Soil Sci Plant Nutr 21 , 104–118 (2021). https://doi.org/10.1007/s42729-020-00346-3

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Water’s essential function as drinking water is a significant daily intake. Contamination by microorganisms (bacteria or viruses) on water sources and drinking water supplies is a common cause in developing countries like Indonesia. This paper will discuss the sources of clean water and drinking water and their problems in developing countries; water quality and its relation to public health problems in these countries; and what efforts that can be make to improve water quality. The method used is a literature review from the latest journals. Water quality is influenced by natural processes and human activities around the water source Among developed countries, public health problems caused by low water quality, such as diarrhea, dysentery, cholera, typhus, skin itching, kidney disease, hypertension, heart disease, cancer, and other diseases the nervous system. Good water quality has a role to play in decreasing the number of disease sufferers or health issues due to drinking and the mortality rate. The efforts made to improve water quality and public health are by improving WASH (water, sanitation, and hygiene) facilities and infrastructure and also WASH education.

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Moringa oleifera , also known as the “tree of life” or “miracle tree,” is classified as an important herbal plant due to its immense medicinal and non-medicinal benefits. Traditionally, the plant is used to cure wounds, pain, ulcers, liver disease, heart disease, cancer, and inflammation. This review aims to compile an analysis of worldwide research, pharmacological activities, phytochemical, toxicological, and ethnomedicinal updates of Moringa oleifera and also provide insight into its commercial and phytopharmaceutical applications with a motive to help further research. The scientific information on this plant was obtained from various sites and search engines such as Scopus, Pub Med, Science Direct, BMC, Google Scholar, and other scientific databases. Articles available in the English language have only been referred for review. The pharmacological studies confirm the hepatoprotective, cardioprotective, and anti-inflammatory potential of the extracts from the various plant parts. It was found that bioactive constituents are present in every part of the plant. So far, more than one hundred compounds from different parts of Moringa oleifera have been characterized, including alkaloids, flavonoids, anthraquinones, vitamins, glycosides, and terpenes. In addition, novel isolates such as muramoside A&B and niazimin A&B have been identified in the plant and have potent antioxidant, anticancer, antihypertensive, hepatoprotective, and nutritional effects. The traditional and nontraditional use of Moringa, its pharmacological effects and their phytopharmaceutical formulations, clinical studies, toxicity profile, and various other uses are recognized in the present review. However, several traditional uses have yet to be scientifically explored. Therefore, further studies are proposed to explore the mechanistic approach of the plant to identify and isolate active or synergistic compounds behind its therapeutic potential.

1. Introduction

Moringa oleifera ( M. oleifera ), the “miracle tree”, thrives globally in almost all tropical and subtropical regions, but it is believed to be native to Afghanistan, Bangladesh, India, and Pakistan [ 1 ]. The Moringa family comprises 13 species ( M. oleifera , M. arborea , M. rivae , M. ruspoliana , M. drouhardii , M. hildebrandtii , M. concanensis , M. borziana , M. longituba , M. pygmaea , M. ovalifolia , M. peregrina , M. stenopetala ), of which M. oleifera has become well known for its use in nutrition, biogas production, fertilizer, etc., [ 2 , 3 ]. Moringa has the unique property of tolerating drought [ 3 ]. Studies have shown that M. oleifera is among the cheapest and most reliable alternatives for good nutrition [ 4 ]. Nearly all parts of the tree are used for their essential nutrients. M. oleifera leaves have a high content of beta-carotene, minerals, calcium, and potassium [ 5 ]. Dried leaves have an oleic acid content of about 70%, which makes them suitable for making moisturizers [ 6 ]. The powdered leaves are used to make many beverages, of which “Zija” is the most popular in India [ 7 ]. The bark of the tree is considered very useful in the treatment of different disorders such as ulcers [ 8 ], toothache [ 9 ], and hypertension [ 10 ]. Roots, however, are found to have a role in the treatment of toothache [ 9 ], helminthiasis [ 11 ], and paralysis [ 12 ]. The flowers are used to treat ulcers, enlarged spleen, and to produce aphrodisiac substances [ 2 ]. The tree is believed to have incredible properties in treating malnutrition in infants and lactating mothers [ 3 ]. The present review aims to sum up the updated insight regarding the pharmacological activities, worldwide research analysis, toxicological, phytochemical, and ethnomedicinal properties of M. oleifera .

2. Material Method

2.1. article eligibility criteria.

In the framework of searching for study material, the following keywords were used: “ Moringa oleifera ”, “pharmacology M. oleifera ”, “phytochemistry M. oleifera ”, “ethnobotanical applications M. oleifera ”, “toxicology M. oleifera ”, and other combinations of terms such as biochemical constituents, taxonomic classification, geographical distribution, and plant formulation to search relevant peer-reviewed journals in various scientific databases such as Scopus, PubMed, Springer, Google scholar, and Wiley. Articles available in the English language have only been referred for review. Articles were analyzed by reading the title and abstracts of the articles found, which clearly indicated that they were all relevant.

2.2. Software and Techniques Used

Chemical structures identified in the plants were searched in the Webbook, Chemspider, and PubMed databases, and at the same time, the identified structures were drawn using Chem Draw (version 12.0.2). VOS Viewer software (1.6.18) was used to generate the map of global collaboration between countries. Global country boundaries (GIS layers) were obtained from an open-source web platform (DIVA-GIS) for geographic mapping. Spatial techniques were used with ArcGIS 10.1 to map M. oleifera indigenous and introduced countries and research activities in each country.

3. Worldwide Research and Collaboration

A country-specific research database on M. oleifera was extracted from Scopus, and the data were linked directly to the country shapefile using the “connect field” function in the GIS environment for geospatial mapping. The analyses revealed that scientists from about 15 countries had published more than 100 research papers during the period 2000–2022. Among which India was the most prolific country ( n = 1083), followed by Nigeria ( n = 441), Brazil ( n = 383), Egypt ( n = 361), China ( n = 331), Indonesia ( n = 327), Pakistan ( n = 281), South Africa ( n = 272), Malaysia ( n = 260), the United States ( n = 214), Saudi Arabia ( n = 205), Mexico ( n = 163), Thailand ( n = 127), and Italy ( n = 105), ( Figure 1 ).

ArcGIS 10.1-based spatial distribution map highlights research papers published on M. oleifera worldwide. A spatial technique was used to generate a map, and GIS layers were obtained from DIVA-GIS, an open-source web platform.

International studies on M. oleifera involve the collaboration of scientists from countries such as India, China, Egypt, Saudi Arabia, Cuba, Australia, the United States, Nigeria, and Portugal, which is indicated by circles in the map ( Figure 2 ). The analysis shows that many articles were published in the past decade, indicating the growing interest of researchers in this plant worldwide.

Network visualization of international collaborative research conducted for M. oleifera using VOS viewer (1.6.18).

4. Taxonomical Classification

The plant M. oleifera belongs to the Kingdom: Plantae; Sub kingdom: Tracheobionta; Super division: Spermatophyta; Division: Magnoliophyta; Class: Magnoliopsida; Sub class: Dilleniidae; Order: Capparales; Family: Moringaceae; Genus: Moringa; Species: oleifera [ 3 , 13 , 14 ].

5. Morphology

The tree grows rapidly in loamy and well-drained sandy soils, preferring a height of 500 m above sea level [ 1 ]. Normally, the tree is small to medium in size, the leaves are naturally trifoliate, the flowers are born on an inflorescence 10–25 cm long [ 14 ], and the fruits are usually trifoliate and commonly referred to as “pods” [ 3 ]. The trunk usually grows straight but is occasionally poorly formed, the branches are usually disorganized, the canopy is umbrella-shaped; the brown seeds have a semi-permeable hull, and each tree has a capacity of about 15,000–25,000 seeds per year [ 10 ].

6. Botanical and Geographical Distribution

M. oleifera is widely distributed worldwide, but its indigenous origin is in India, Arabia and the East Indies. It is common in Asia, Africa, the Caribbean, Latin America, the Pacific Islands, Florida, Madagascar, Central America, Cuba, the Philippines, Ethiopia, and Nigeria [ 2 , 15 ]. The history of the plant explains that M. oleifera was introduced from India to Africa, Southeast Africa, and the Philippines in ancient times [ 16 , 17 ] ( Figure 3 ). It requires tropical and subtropical regions and grows at a temperature of about 25–35 °C [ 1 ]. M. oleifera is a deciduous type of tree typically grown in tropical and subtropical regions across the globe [ 18 , 19 ]. It grows best in indirect sunlight and without waterlogging, and the soil should be slightly acidic to alkaline. The tree begins to bear fruit at 6 to 8 months of age [ 18 ]. Commercially, it is grown in different countries such as Africa, Mexico, Hawaii, and South America, but due to different soil conditions, the nutrient content varies from country to country [ 3 ].

ArcGIS 10.1-based spatial distribution map of Moringa oleifera, the purple color shows the indigenous countries like India, Saudi Arabia, and East indies, whereas the green color shows the introduced countries and regions such as Tropical Asia, Latin America, Africa, Pacific Island, Caribbean Florida, Madagascar, Central America, Cuba, Philippines, Ethiopia, and Nigeria. GIS layers were obtained from DIVA-GIS, an open-source web platform.

7. Ethnomedicinal/Traditional Properties

People worldwide have included M. oleifera in their diet since ancient times because of its vital therapeutic values ( Table 1 ). Various medicines made from the plant are said to have ethnomedicinal properties for curing diseases and have been used for centuries. Approximately every part (leaf, pod, bark, gum, flower, seed, seed oil, and root) of this plant has been used to treat one disease or another [ 20 ]. Uses of M. oleifera are observed in pathological alterations such as antihypertensive [ 10 ], anti-anxiety [ 21 ], anti-diarrheal [ 22 ], and as a diuretic [ 23 ]. Moringa is also used to treat dysentery [ 24 ] and colitis [ 25 ]. A poultice made from Moringa leaves is a quick remedy for inflammatory conditions such as glandular inflammation, headache, and bronchitis [ 9 ]. The pods treat hepatitis and relieve joint pain [ 19 ]. The roots are conventionally used to treat kidney stones [ 26 ], liver diseases [ 27 ], inflammation [ 28 ], ulcers [ 29 ], and pain associated with the ear and tooth [ 30 ]. The bark of the stem is used to treat wounds and skin infections [ 31 ]. Indians use the gum extracted from this plant to treat fever, and it is also used to induce abortions [ 32 ]. The seeds of the plant act as a laxative and are used in the treatment of tumors, prostate, and bladder problems [ 33 ]. The seeds show promise for the treatment of arthritis by altering oxidative stress and reducing inflammation [ 34 ]. Preparations from the plant leaves benefit nursing mothers and malnourished infants and improve the general health of the population. The leaves have been useful for patients suffering from insomnia [ 35 ] and treating wounds [ 36 ]. Moringa is used incredibly extensively in the cosmetic industry nowadays, and in ancient Egyptian history, it was similarly used for preparing dermal ointments [ 37 ].

Uses of Moringa oleifera listed in Ayurvedic medicinal textbook.

8. Pharmacological Uses

Recent pharmacological studies have revealed that different extracts of M. oleifera exhibit different pharmacological activities, such as antimicrobial [ 43 ], antifungal [ 44 ], anti-inflammatory [ 45 ], antioxidant [ 46 ], anticancer [ 47 ], fertility [ 48 ], wound healing [ 43 ], and other pharmacological activities mentioned below ( Table 2 ).

Phytoconstituents of Moringa and their relevant therapeutic effects.

8.1. Antimicrobial and Antifungal Activity

M. oleifera ethanolic root extract contains a compound N-benzylethyl thioformate (an aglycone of deoxyniazimincin) responsible for the antimicrobial and antifungal effect toward an extensive array of microbes and fungi [ 44 ]. M. oleifera methanolic leaf extract may exert inhibition of urinary tract infections caused by Gram-negative and Gram-positive bacteria such as Klebsiella pneumoniae , Staphylococcus aureus , Escherichia coli , and Staphylococcus saprophyticus [ 69 ].

The inhibitory effect of extracts from leaves, seeds, and stems of M. oleifera has been specified in various fungal strains such as Aspergillus flavus, Aspergillus terreus, Aspergillus nidulans, Rhizoctonia solani, Aspergillus niger, Aspergillus oryzae, Fusarium solani, Penicillium sclerotigenum, Cladosporium cladosporioides, Trichophyton mentagrophytes, Penicillium species, Pullarium species [ 44 ]. M. oleifera seeds have active components 4-(alpha-L-rhamanosyloxy) benzyl isothiocyanates, which are believed to be responsible for their antimicrobial activity [ 70 ]. The juice of Moringa leaves also showed potential against human pathogenic bacteria [ 43 ]. The methanolic leaf extract has nearly 99% inhibition against Botrytis cinerea (a necrotrophic plant fungus) [ 71 ].

The fruit of M. oleifera contains alkaloids, flavonoids, and steroids, which have an inhibitory effect against the culture of Candida albicans by either denaturing the protein or inhibiting the germination of spores through the steroid ring they contain [ 72 ].

Strong inhibitory effects of moringa seed kernel extract were observed for Bacillus cereus , Staphylococcus aureus , Mucor species , and Aspergillus species . However, it was less effective against P. aeruginosa and E. coli . This indicated that, except for E. coli and P. aeruginosa , moringa seed kernel extract might be utilized to treat infections caused by these species [ 73 ]. A recent study has been conducted, which states that only apolar extract obtained from seeds of M. oleifera showed anti-microbial activity against Gram-positive bacteria [ 74 ].

8.2. Anti-Inflammatory Activity

A significant anti-inflammatory effect was observed in different parts of M. oleifera (leaf, pods, flowers, and roots). It was observed that the isolated compound (4-[2-o-Acetyl-alpha -l-rahamnoslyloxy) benzyl] thiocynate from Moringa possessed nitric oxide inhibitory activity and was subsequently found to be effective in Raw264.7 cell lines [ 75 ]. A compound derived from M. oleifera roots, known as aurnatiamide acetate and 1,3-dibenzylurea, inhibited TNF-α production [ 76 ]. Active compounds such as tannins, phenols, alkaloids, flavanoids, carotenoids β-sitosterol, vanillin, and moringin have anti-inflammatory properties [ 32 ]. The M. oleifera fruit extract blocked nuclear factor kappa B (NF κB) translocation, and the chloroform extract was found to be cytotoxic at high concentrations (500–1000 µg/mL) [ 77 ]. M. oleifera leaves extract was used in mice for treating atopic dermatitis in human keratinocytes and was found to be effective in reducing the expression of mannose receptor mRNA, thymic stromal lymphopoietin, and retinoic acid-related orphan receptor γT in ear tissues ( Figure 4 ) [ 78 ].

M. oleifera , as an oxidative and inflammatory marker, inhibits IKBα phosphorylation, thereby preventing NFKB (nuclear factor kappa B) inhibition. It prevents the nuclear translocation and dimerization of IkBα and NFKB, thereby inhibiting the formation of inflammatory proteins such as TNFα(tumor necrosis factor), COX-2(cyclooxygenase-2), IL6(interleukin -6), and iNos(inducible nitric oxide synthase) and thereby reducing the inflammation and curing other disorders like obesity, arthritis, cancer, diabetes, and ulcer.

8.3. Oxidative Stress

The results of M. oleifera were observed in methotrexate-induced mice. The study aimed to look into a probable palliative effect of M. oleifera extract on mice. The mice received the extract one week before administering methotrexate injection, and this treatment was continued for 12 days. The result showed that pretreatment with an extract of M. oleifera on mice poisoned with methotrexate could protect them from oxidative stress [ 79 ].

The antioxidant activity of ethanolic extract M. oleifera stems exhibited a protective effect against epidermal oxidative stress injury induced by H 2 O 2 in keratinocytes. The result displayed that the stems showed antioxidant potential, and, therefore, can be used as an excellent and preventive source in animal epidermal oxidative stress injury [ 80 ].

The research investigated the antioxidant potential of Moringa leaves against diclofenac sodium-induced liver toxicity in animals. The researchers concluded that the extract was significantly effective against diclofenac-induced liver toxicity and, therefore, can be considered liver protective [ 81 ].

8.4. Anti-Oxidant Activity

Bioactive compounds such as glycosylates [ 82 ], isothiocyanates [ 62 ], thiocarbamates [ 83 ], flavonoids [ 84 ], and certain other compounds from Moringa pods have been investigated for reactive oxygen spices. The aqueous extract has been shown to be a potent free radical scavenger against free radicles [ 45 ]. Previous studies suggest that the antioxidant potential might be due to kaempferol, which is mainly found in plant leaves [ 43 ]. The synergistic outcome of Moringa was observed with piperine and curcumin on oxidative stress induced by beryllium toxicity in Wistar rats [ 85 ]. The alcoholic extract of the plant reduced glucose-induced cataractogenesis in isolated goat eye lenses by controlling GSH levels [ 86 ]. Myricetin, derived from the Moringa seed extract, has proved to be a better antioxidant than BHT (butylated hydroxytoluene) and alpha-tocopherol. M. oleifera leaf extract and compounds, such as isoquercetin, astragalin, and crypto-chlorogenic acid, help lower ROS in HEK-293 cells [ 87 ]. Moringa is also helpful in reducing plasma monoaldehyde (MDA) levels in fasting plasma glucose (FPG) concentration in healthy volunteers compared to the individuals fed with warm water. A dose-dependent upsurge in GSH and reduced MDA levels were observed with alcoholic extract of the plant without toxic effect till 100 mg/kg ( Figure 4 ) [ 46 ].

8.5. Anti-Cancer Activity

Several parts of moringa (fruits, leaves, flowers, stems) have been shown to be beneficial against cancer, a deadly disease. The isolated compounds thiocarbamate and isothiocyanate from moringa act as inhibitors of tumor cells [ 15 , 47 ]. The dichloromethane fraction was found to be cytotoxic for MCF7 breast cancer cells [ 88 ]. Niazimincin has been projected as an effective chemopreventive agent in chemical carcinogenesis [ 44 ]. Alcoholic and hydro-methanolic extracts of fruits and leaves have shown significant tumor growth retardation in the melanoma mouse model [ 32 ]. Soluble cold distilled water from Moringa inhibited tumor cell growth and reduced ROS (reactive oxygen species) in cancer cells [ 45 ]. A recent study based on computational modelling suggests that M. oleifera contains rutin with the highest binding affinity with BRAC-1 (Breast Cancer Gene-1) [ 49 ].

8.6. Fertility and Anti-Fertility Activity

Adding to the list of beneficial effects of Moringa, the various parts of the plant possess fertility and abortion-inducing properties. The aqueous extract at a 200 and 400 mg/kg dose has been found to be more abortifacient and anti-fertility effects [ 44 ]. Recent studies on hot and cold extracts of leaves of M. oleifera propose that ingestion of Moringa before, after, and during pregnancy may lead to adverse fetal developmental outcomes by causing rigorous contraction of the uterine wall [ 89 ].

8.7. Hepatoprotective Activity

Among the numerous flavonoids (quercetin, kaempferol, isoquercetin, rhamnetin, etc.,) present in Moringa, quercetin in Moringa flowers is thought to be accountable for the hepatoprotective effect [ 44 ]. Methanolic extract at low dose showed changes in hepato-renal and hematological profile with significant changes in serum aminotransferase concentration, plasma cholesterol level, alkaline phosphate, bilirubin, and serum LPO levels. However, the higher dose of the extract altered total bilirubin, blood urea nitrogen, and non-protein nitrogen levels and decreased the clotting time [ 43 ]. Liver injuries induced by acetaminophen in Sprague-Dawley rats, where the standard drug taken was silymarin, Moringa showed similar hepatoprotective properties in these rats by dropping the levels of AST, ALT, and ALP [ 90 ]. The seeds were also found to be effective against carbon tetrachloride-induced liver fibrosis, as evidenced by a reduction in serum aminotransferase activity and globulin levels [ 91 ]. Treatment with this plant extract for about 21 days regularly as diet significantly reduced liver injury, and this effect was found due to alkaloid, quercetin, kaempferol, flavonoids, ascorbic acid, and benzyl glucosinolates in this plant [ 32 ].

8.8. Cardiovascular Activity

The freeze-dried aqueous and alcoholic extract of M. oleifera showed cardioprotective activity in animals induced with myocardial infarction by isoproterenol [ 92 ]. Chronic treatment of M. oleifera was effective on isoproterenol-induced hemodynamics and improved the levels of enzymes such as SOD, catalase, lactase dehydrogenase, glutathione peroxidase, and creatine kinase [ 92 ]. Butanolic extract has been proven to be a rich antioxidant source in rats with cardiac necrosis induced with isoproterenol. Moreover, it was found to significantly reduce inflammatory levels and myocardial necrosis due to the presence of the compound N-α-rhamnopyranosyl vincosamide [ 93 ]. Moringa leaves significantly lowered cholesterol levels by showing a protective effect on hypertensive rats. The active constituents believed to be responsible for this activity were identified as niazirmin A, niazirimin B, and niazimincin [ 32 ].

8.9. Anti-Ulcer/Gastroprotective Activity

Bisphenols and flavonoids found in moringa leaves showed a reduced level of ulcer index, duodenal ulcer, and stress ulcer in the ibuprofen-induced gastric ulcer model [ 32 ]. Moringa extract was shown to significantly reduce free radicals and neutralize the acidic behavior of gastric juice and have a protective effect on the development of gastric ulcer [ 94 ]. The presence of flavonoids in the plant has been shown to have a protective effect on ulcer formation by increasing capillary resistance and improving microcirculation, resulting in less cell injury [ 95 ].

8.10. Analgesic/Antipyretic Activity

Moringa leaf extract shows analgesic activity in almost all tree parts in central and peripheral animal models [ 32 ]. Multiple factions of alcoholic extracts such as petroleum ether, n-butanol, ethyl acetate, and dimethyl ether were found to have potent analgesic activity compared to standard aspirin [ 96 ]. At a dosage of 30–300 mg/kg, the polar and non-polar extract of leaves showed a remarkable drop in prostaglandin and bradykinin levels compared to the seed and root extract in a nociceptive study of formalin-induced paw edema [ 96 ]. The ether and ethyl acetate fractions of the seeds were studied in a hyperpyrexia model [ 97 ], keeping paracetamol as standard 200 mg/kg, and the extract proved to have the best antipyretic activity among all [ 45 ].

8.11. Neuropharmacological Activity

Previous results have proved that leaves extract reestablishes levels of monoamine in the brain and is very helpful in Alzheimer’s disease, while the in vitro activity of the ethanolic extract of the leaves showed an anticonvulsant effect on dopamine and norepinephrine levels, locomotor activity, and serotonin (5HT) in the brain in penicillin-induced convulsions [ 98 , 99 ]. The methanolic root extract in mice induced by pentobarbital sodium and diazepam has remarkable sedative effects on the CNS by improving sleep duration [ 35 ]. The toluene acetate fraction of the methanolic extract proved its potency as a possible nootropic agent [ 97 ]. The leaves have shown good anticonvulsant activity in a phenyl tetrazoline and maximal electric shock-induced model using male albino mice [ 98 ]. The aqueous extract of the roots blocked the epileptic seizures induced by penicillin in adult albino rats [ 99 ]. The ethanolic leaves extract exhibited anxiolytic properties, which were confirmed in behavioral experiments using the actophotometer and the rotarod device, respectively ( Figure 5 ) [ 32 ].

The various phytoconstituents present in M oleifera are responsible for numerous neuroprotective effects. M. oleifera is responsible for upregulating synaptic activity, cholinergic activity, dopaminergic activity, signaling of NrF2 (Nuclear factor erythroid 2-related factor 2), and simultaneously decreasing beta-amyloid toxicity and phosphorylation of tau proteins.

8.12. Neuropathic Pain

The broad spectrum of phytoconstituents of the leaves extract of Moringa has led researchers to develop a herbal alternative for treating chronic neuropathic pain caused by constriction. The need to limit conventional analgesics for this disease. Diabetic rats inflicted with neuropathic pain caused by chronic constriction were used for the study. Tests conducted before and after treatment with moringa leaves showed that they significantly altered the neuropathic pain condition in diabetic rats. It suggests that the drop in oxidative stress might be the underlying mechanism in treating neuropathic pain and thus could be used as an effective novel source for the same [ 100 ].

A research team explored the bio-guided fractions of Moringa seed extract on a diabetes-induced neuropathic pain model. After conducting various oxidative and other experimental studies on induced and treated rats, the team concluded that the extract-treated rats exhibited reasonable glycemic control and antinociceptive properties and proved to be a powerful neuroprotective agent with a high margin of safety ( Figure 5 ) [ 101 ].

8.13. Wound Healing Effect

A significant effect in studies on wound healing after incision or excision was demonstrated for ethyl acetate, and water extract of M. oleifera leaves at a 300 mg/kg dose [ 43 ]. Studies reported that in preclinical studies, leaves, seeds, and dried pulp extracts have shown effective enhancement of wound closure, granuloma rupture strength, and reduction of skin rupture strength in the scar area [ 102 ]. Leaf extracts have shown promising results in diabetic animals by improving the downregulation of inflammatory markers and increasing the vascular endothelial growth factor level in the injured tissue [ 32 ]. Compounds present in aqueous extract have shown a considerable effect on diabetic foot ulcers by downregulating the levels of various inflammatory markers [ 102 ]. The researcher conducted an in vitro assay to select the standardized extract with the highest potency, which was then converted into a film for wound healing. The result showed that the aqueous extract had the maximum cell proliferation and migration properties among the different extracts [ 103 ].

8.14. Immunomodulatory Activity

Methanolic extract of the plant contains active constituents such as isothiocyanate and glycoside cyanide, which exhibit immunostimulatory activity and effectively enhance immunity. The recent review paper suggests that various bioactive compounds have been used to treat various immune-related disorders such as cancer, hypertension, and diabetes, thereby enhancing host immunity [ 104 ].

8.15. Hematological Activity

M. oleifera has demonstrated its significant benefits in hematological activities. A randomized, double-blind study suggests that aqueous leaf extract effectively improves women’s low hemoglobin levels (8–12 g/dL) [ 105 ]. Another study showed that M. oleifera leaves, when taken for 14 days by healthy volunteers, significantly improved platelet counts [ 32 ].

8.16. Anti-Obesity Activity

In a study, oral treatment with leaf powder of M. oleifera for a duration of nearly 49 days was found to significantly reduce body mass index (BMI) in rats suffering from hypercholesterolemia [ 106 ]. The mechanistic approach behind this was the downregulation of mRNA expression of the hormones resistin and leptin and the concomitant increase in regulation of the gene adiponectin in rats [ 32 ]. A recent study revealed the mechanistic approach for the anti-obesity effect of M. oleifera . The plant significantly improved lipid profile by reducing body weight. It also regulated adipogenesis-related genes, increased glucose tolerance, and decreased levels of hormones such as vaspin, leptin, and resistin ( Figure 6 ) [ 107 ].

M. oleifera as a promising anti-obesity agent. Various in-vitro findings suggest that supplements of M. oleifera cause direct inhibition of pancreatic lipase, thus reducing the conversion of triglycerides into simple. Moringa has fat storage regulation by upregulation of lipolysis-associated protein and down-regulating the expression of protein related to fat storage. It is also effective in the improvement of antioxidant levels. Besides these, Moringa is also responsible for increasing ghrelin levels and decreasing leptin, producing a feeling of satiety.

8.17. Anti-Allergic Activity

The ethanolic seeds extract reduced histamine release and also suppressed the anaphylaxis induced by anti-immunoglobulin G. The mechanism underlying this effect may be the membrane-stabilizing potential of mast cells in an oval albumin sensitization model [ 32 ].

8.18. Anti-Diabetic Activity

Moringa leaves showed excellent results in the glucose tolerance of Wistar and Goto-Kakizaki rats and also lowered blood glucose levels. The aqueous extract showed an antidiabetic effect in rats by controlling blood glucose levels, protein, sugar, and hemoglobin [ 45 ]. The leaves of the plant were found to lower glucose levels within three hours of intake, but not more than the standard drug glibenclamide. Moringa seeds, when administered orally, contain insulin-like proteins that have antigenic epitopes such as insulin and exhibit antihyperglycemic activity [ 108 ]. Leaf extracts of the plant also have antidiabetic activity as they increased CAT and MDA levels, reduced FPG levels, hemoglobin levels, LDL-C, and VLDL-C in type 2 diabetic patients and, most importantly, increased insulin levels in healthy subjects [ 109 ]. The seed extract of the plant reduced LPO levels and amplified the antioxidant effect in mice induced with streptozotocin, the seed extract was also able to reduce IgG, IgA, and IL -6 parameters and pancreatic β-cell activity, and it was suggested that the bioactive compound responsible for this effect were quercetin, kaempferol, glucomoringin, chlorogenic acid, and isothiocyanates [ 110 ].

8.19. Diuretic Activity

The alcoholic and aqueous root extract of M. oleifera significantly affects calcium oxalate urolithiasis in male rats. This reduction was observed due to the decrease in the retention level of oxalates, calcium and phosphates as well as serum urea nitrogen, creatinine, and uric acid [ 23 ].

8.20. Angiotensin Converting Enzyme (ACE) Activity

Compounds such as niazimin-A, niazicin-A, and niaziminin-B are stated to be present in the M. oleifera plant extract. These compounds were found to have potent antihypertensive activity when targeted to (ACE), an important enzyme of the renin-angiotensin system. The researchers observed this activity by protein–ligand docking and found that the compounds have a high affinity for the angiotensin-converting enzyme compared with captopril and enalapril (standard drug) [ 111 ]. The angiotensin enzyme rennin plays a prominent role in regulating blood pressure and leading to diseases such as hypertension, kidney disease, and other cardiovascular diseases. The study found that the role of M. oleifera with two other plants ( Azadirachta indica and Hibiscus sabdariffa ) inhibited the enzyme with percentage inhibition (71.8%, 74%, and 73.4%) compared to standard drugs (captopril and enalapril). The compound responsible for this activity of Moringa was termed β-sitosterol [ 112 ].

8.21. Anti-Venom Effect

The leaves of the plant extract have been shown to be effective against the venom of Naja Nigricollis (a snake species) in rats. This snake’s venom contains potent neurotoxins that cause the degradation of phospholipids at the plasma membrane, affecting the normal neurotransmission process and causing hemolysis and hemorrhage. The results showed that Moringa extract effectively cured acute anemia, and a remarkable increase in micronucleated polychromatic erythrocytes was observed in rats treated with M. oleifera [ 113 ].

8.22. Cytotoxicity Effect

The cytotoxic potential of M. oleifera on human mesenchymal myeloma cell lines is observed in methanolic extract. The results of the extracts showed a higher ID50 value than other extracts. The researchers also found that the alkaloids and flavonoids contained in the plant showed some similarity to vincristine and vinblastine by random experiments. Therefore, the plant can be recommended for the herbal treatment of myeloma patients [ 47 ].

It was found that the ethanolic leaves extract of M. oleifera contains active constituents that can alleviate cyclophosphamide-induced testicular toxicity by promoting genes associated with the functional integrity of spermatozoa and enlargement of DNA in spermatogonia. Therefore, the administration of the extract not only improved blood and intestinal hormone levels but also modulated the expression of genes responsible for Sertoli and spermatogonial cells [ 114 ].

9. Toxicity

Various experimental procedures were conducted to evaluate the toxic potential of the plant. A random selection of female non-pregnant Wistar albino rats was conducted with an oral dose of 2000 mg/kg aqueous methanol solution. Blood samples were collected, and the ALT, AST, and total bilirubin content were determined. The outcome of the study suggested that the lethal dose of the aqueous extract was higher than 2000 mg/kg in female rats [ 53 ].

A similar study was also conducted in Sprague-Dawley rats to evaluate the acute toxic potential of Moringa leaf powder. The experiment also found that oral administration of dried leaves up to 2000 mg/kg had no harmful or lethal effect on the human body [ 115 ].

The toxicity of M. oleifera seeds in rats was observed at acute and subacute levels (methanolic extract). Acute toxicity was seen at a dose of 4000 mg/kg, whereas mortality was observed at a dose of 5000 mg/kg. Therefore, in a nut shell, it could be summarized that the seed extract could be safe for nutritional use [ 116 ].

Experiments conducted for acute and subacute studies indicated that the stem bark extract did not cause any toxic effect in the acute and subacute toxic studies up to 2000 mg/kg. Therefore, the researchers concluded that the stem bark of M. oleifera can be considered non-toxic when administered orally [ 117 ].

The subacute toxicity test at a dose of 250, 500, and 1500 mg/kg was performed for 60 days. The result showed that the lethal dose was 1585 mg/kg without significant alterations in sperm quality, biochemical, and hematological parameters compared to the control group [ 105 ].

The acute toxic study (5000 mg/kg) and subacute (40–1000 mg/kg) results showed no adverse reaction during these studies. However, increased ALT, ALP, and lower creatinine levels were observed. Therefore, it could be concluded that consumption is safe, but intake should not surpass 70 gm/day to prevent cumulative toxicity [ 118 ].

10. Clinical Trials

To date, 25 clinical studies have been conducted on M. oleifera , fifteen of which have been completed. Nine of these fifteen studies addressed M. oleifera as part of a diet, while the remaining studies were limited to disease-specific drug interventions. Overall, the studies demonstrated the efficacy of using moringa for conditions such as malnutrition, chronic kidney disease, HIV infection, and reproductive health [ 119 ].

A clinical study highlighted the significant role of M. oleifera as an anti-asthmatic agent. In this study, researchers used seed kernels of M. oleifera to treat symptoms of bronchial asthma. Candidates were selected based on inclusion and exclusion criteria, with respiratory parameters and blood samples recorded before and at the end of three weeks of treatment with M. oleifera . The extract was administered to patients in the form of dried powder at a dose of 3 g twice daily for three weeks, and they were instructed to take water with the powder. Symptoms were graded as severe, moderate, and mild on a point table. The results indicate that symptoms and respiratory functions decreased; M. oleifera seeds can effectively treat bronchial asthma [ 120 ].

11. Phytopharmaceutical Formulations

Plant extracts have always attracted researchers’ attention for producing various pharmaceutical products. This process usually involves the production of medicinal products characterized by two things: first, the production of a stable product, and second, patient compliance. The advantage of Moringa plant extracts is that it appears to be exceedingly safe at the doses and in the amounts commonly utilized for therapeutic efficacy [ 20 ]. M. oleifera has been widely accepted in the research area, and scientists have used an array of approaches to develop various formulations. The various phytoformulations prepared using M. oleifera are tabulated below ( Table 3 ).

Phytopharmaceutical formulations prepared using M. oleifera extract.

12. Miscellaneous Uses

A study was performed on M. oleifera using the HPLC-based cyclo condensation method. Astragalin and isothiocyanates were used as markers for standardization of the plant. The conclusive result of the study suggests that the standardized method might be useful for assessing the quality of the development of cosmetic and natural health products [ 144 ].

The extract of M. oleifera leaves was helpful in eliminating the adverse effects of neem oil, which is used in aquaculture as an insecticide to control predators and parasites of fish fry. The researchers concluded that the extract of M. oleifera leaves eliminated the oxidative stress and toxicity caused by neem oil [ 145 ].

The lower yield of okra (Abelmoschus esculentus) was studied. The low production of these crops was due to infestation by pests and insects and poor soil nutrient content. In order to improve production conditions, different chemical pesticides were used, which brought further environmental risks. The use of M. oleifera aqueous leaf extract at different concentrations (1:30 and 1:40) proved beneficial for okra [ 146 ].

The efficacy of M. oleifera leaf and root extract was evaluated as a plant growth regulator and biopesticide in the wheat harvest. The researcher used different concentrations (5, 10, 12.5, 25% w / w , w / v , v / v ) of Moringa leaf and root extract at different stages of the wheat plant. Significant plant growth was observed, resulting in increased yield and a decrease in aphid invasion [ 147 ].

M. oleifera is rich in macronutrients and micronutrients, vitamins, phytohormones, alkaloids, and flavonoids, which make this plant a multipurpose plant. Recent research has shown that Moringa extract is also helpful in tolerance to abiotic and biotic stress under stressful environmental conditions [ 148 ].

The therapeutic effect of bioactive constituents (flavonoids, alkaloids, tannins, isothyocyanin and beta-sitosterol) present in M. oleifera has been reported in chronic diseases such as hyperlipidemia [ 149 ], hypertension [ 150 ], hepatoprotective [ 151 ], anti-cancer [ 152 ], Alzheimer’s disease [ 153 ], Parkinson’s disease [ 154 ].

The combined effect of M. oleifera and praziquantel in rats was studied by a group of researchers. The seeds and leaves of M. oleifera were considered to evaluate their bioavailability with praziquantel and also the in vivo effects of the same were observed on Taenia crassiceps. The study showed that the combined action of both had a significant amount of cytocidal activity compared to the rats, which were only administered with praziquantel [ 155 ].

A variety of bioactive nutrients, such as flavonoids and vitamins, is available in the M. oleifera plant. The in vitro study conducted by a group of researchers focused on bioactive compounds that make up the nutritional potential of plants [ 156 ]. The conclusive statement of the researcher revealed that the high content of proteins, lipids, and sulfur-containing amino acids and the relative lack of toxic components make moringa a great nutritional alternative for humans [ 157 ].

The bioactive isolate palmitic acid from the leaf extract of Moringa has been attributed to broad therapeutic benefits. A team of researchers studied this isolate against a wide range of microbial and fungal strains. The results showed that it had the highest zone of inhibition for both fungal and microbial strains [ 4 ].

The polyphenols and flavonoids present in M. oleifera to scavenge free radicals could be useful in developing an anticancer drug delivery system. Nanoparticle technology was used to incorporate Moringa extract as a drug carrier. Treatment of HeLa cell lines with a single dose of the plant showed that the composite film of the plant extract was efficient in killing malignant cells compared to other isolated and purified phytocomponents on the market [ 158 ].

The bioactive components of M. oleifera inhibit the inflammatory markers in lipopolysaccharide-induced human macrophages. The induced macrophages were treated with M. oleifera extract. Thereafter the treated cells were tested for their anti-inflammatory and cellular mechanism. The results revealed that the extract suppressed mRNA expression of IL -6, IL -1, NF-κB (P50), PTGS2, and TNF-α. At the same time, the inhibition of phosphorylation of IκB-α and nuclear factor (NF)-κB was also observed in the study. The researchers’ final statement suggests that the blocking of NF-κB and IκB-α may be the reason for the inhibition of inflammation [ 159 ].

Apart from its wide use in preventing and curing various human diseases, Moringa is known for a number of non-medicinal uses, chief among which is its use for poultry, especially in curing viral infections (Newcastle Disease Virus) and other parasitic and bacterial diseases that cause mortality in animals [ 160 ]. The plant also serves as an important growth promoter for farmers in the production of tomatoes, peanuts, corn, and wheat in their early vegetative stages [ 161 ]. Environmentally friendly biopesticides are produced from this plant, which is cheap and easily available and helps in curing various plant diseases [ 162 ]. Studies have shown that the total crop production increased by 20–35% by using M. oleifera leaf extract, which is a good sign for increasing agricultural growth at a minimal cost [ 163 ]. The aqueous extract of M. oleifera is a source of various minerals and growth promoters (indole acetic acid, gibberellins, cytokines). It thus can be used as an effective plant biostimulant that could be a simple alternative to the artificial fertilizers and pesticides available in the market [ 161 ]. The methanolic extract of the plant is found to be rich in potassium, calcium, carotenoid, phenols, and zeatin, and when three sprays of this extract are applied on the oilseed rape plant, it is observed that the pods, twigs, height, and number of seeds increase significantly compared to the untreated control group [ 164 ]. The ability of the plant to resist drought is due to the plant hormone “zeatin”, which is present in large quantities in the methanolic extract, so the plants exposed to such climatic conditions when sprayed with the methanolic extract of Moringa showed improved growth characteristics compared with well-watered plants [ 165 ]. The tree is efficient in removing water hardness and is used by African tribes as a cheap source compared to chemical softeners [ 166 ]. A study conducted by several groups found that treating river water in African countries with Moringa seeds reduced color and microorganisms by 90% and microorganism ( Escherichia coli ) levels by up to 95%. Previous reports indicated that a water sample treated with M. oleifera seeds reduced the hardness content of the water by 50–70%, which used to be 80.3 g L −1 CaCO3 [ 167 ]. It has also been shown to be an effective solution for treating turbidity, alkalinity, and dissolved organic carbon. It is suggested that Moringa could be, to some extent, an alternative to chemical alum used to remove water turbidity [ 168 ]. Moringa is a good source for curing plant diseases and can be a good option for biopesticides [ 162 ]. Since various plant pathogens affect the plants, Pythium debaryanum-a pathogen responsible for damping-off disease-can be cured by adding leaves to the soil [ 169 ]. Nearly all plant part (fruits, flowers, leaves, seeds, roots) is believed to have different properties that can heal the body spiritually and psychologically [ 34 ].

13. Phytochemistry

Almost all parts of M. oleifera and its isolates have been studied for research. Based on the literature collected between 2010 and 2022, more than 90 compounds from the genus Moringa have been identified, many of which have therapeutic potential. The isolates fall into the category of proteins and amino acids [ 170 ], phenolic acids [ 171 ], carotenoids [ 172 ], alkaloids [ 173 ], glucosinolates [ 174 ], flavonoids [ 175 ], sterols [ 175 ], terpenes [ 176 ], tannins and saponins [ 177 ], fatty acids [ 178 ], glycosides [ 179 ], and polysaccharides [ 180 ] ( Table 4 and Table 5 ).

Phytochemicals present in different plant part extracts of M. oleifera .

Select phytoconstituents of M. oleifera isolated through various techniques.

ND—not detected; NA—not available; * lower but appreciable amount present; ^ compounds were isolated, but data not available; # the concentration of the constituent may vary according to geographical location.

The leaves contain larger amounts of calcium, potassium, proteins, and amino acids such as arginine and histidine [ 170 ].

M. oleifera leaves contain a higher concentration of phenolic acids and flavonoids, including cinnamic acid, sinapic acid, syringic acid, gentisic acid, gallic acid, ferulic acid, protocatechuic acid, vanillin, caffeic acid, o- coumaric acid, p-coumaric acid and epicatechin are phenolic acids. In contrast, quercetin, catechin, myricetin, and kaempferol fall under the category of flavonoids having excellent therapeutic activity and are listed above in Table 2 [ 171 , 175 , 177 ].

The carotenoid lutein is present in larger quantities in the leaves of M. oleifera [ 172 ]. The therapeutic efficacy of the plant is due to the active constituents present in the plant leaves. The leaves of the plant revealed nearly thirty-five compounds by gas chromatography–mass spectrometry. Among the thirty-five isolated compounds, some important ones isolated in the leaves were palmitoyl chloride, cis-vaccenic acid, 5-O-acetyl-thio-octyl, pregna-7-dien-3-ol-20-one, γ-sitosterol, β-l-rhamnofuranoside, and tetradecanoic acid [ 178 ].

Two new alkaloids, marumoside A and marumoside B, were also isolated from M. oleifera leaves; another type of alkaloid, aurnatiamide acetate was isolated from M. oleifera roots [ 173 , 178 ]. Moringin and moringinine are the two alkaloids present in the plant’s stem [ 179 ].

M. oleifera is a rich source of glucosinolates. The most abundant glucosinolate present in the species is 4-O-(α-L-rhamnopyranosyloxy)-benzyl glucosinolates, also known as glucomoringin [ 174 ].

A sterol isolate, β-sitosterol was found in seeds and leaves of M. oleifera [ 173 ]. Another type of sterol glycoside β-sitosterol-3-O-β-D-galactopyranoside was extracted from M. oleifera bark [ 174 , 175 ].

An interesting category of diterpenes and terpenes is found in M. oleifera leaves. Among which Phytol is diterpene alcohol, a major component of chlorophyll, and is found abundantly in leaves of the plant. At the same time, terpenes and their derivatives are present in trace amounts (linalool oxide, farnsylacetone, isolongifolene α-ionene, and α- and β-ionone), whereas hexahydro-farnesyl acetone can be found in abundance [ 176 ].

Seed oil extract of M. oleifera commonly contains tannins and saponins, which are responsible for numerous pharmacological activities [ 177 ].

Fatty acids such as arachidic acid, octacosanoic acid, oleic acid, palmitic acid, stearic acid, linolenic acid, behenic acid, and paullinic acid are found in M. oleifera seeds [ 178 ].

Two glycosides, namely niazirin and niazirinin were extracted from the ethanolic extract of M. oleifera [ 179 ].

The exudate of the gum contains a large number of poly saccharides such as D-galactose, L-arabinose, D-xylose, L-rhamnose, D-mannose, and -glucuronic acid [ 180 ]. The structure of some of the key phytoconstituents isolated from M. oleifera is shown in Figure 7 .

Structure of some key phytoconstituents isolated from M. oleifera .

14. Current Status

Moringa is a versatile plant with numerous benefits, and the current status of the plant suggests that it can be used in various pharmacological activities and their related formulations, biomedical applications, livestock, poultry, and fish production, enormously. Extensive research conducted in India, Nigeria, Brazil, and China during 2019–2022 has created a valuable resource for researchers worldwide. After an extensive study of this plant, it was found that M. oleifera has evolved to benefit humans in many ways. A large number of nutrients and phytoconstituents in this plant make it suitable for consumption by humans and animals. Due to its high anti-oxidant properties, it has become a pharmaceutical option for the production of formulations such as wound healing, anti-cancer, and anti-ageing etc. It is suitable not only for human use but also as a fertilizer in various forms extracted from M. oleifera . Besides its benefits, it also has severe toxic and abortifacient effects when taken in large quantities.

15. Conclusions and Future Perspective

The review summarizes various aspects of M. oleifera , including its worldwide research, ethnopharmacology, pharmacology activities, phytochemistry, phytopharmaceutical formulations, clinical studies, toxicology, and other miscellaneous parameters. The presence of alkaloids, phenolic acid, glycosides, sterols, glucosinolates, flavonoids, terpenes and fatty acids are responsible for the medicinal effects of M. oleifera . In addition, M. oleifera is also rich in compounds such as vitamins, micronutrients, and carotenoids which increase its medicinal value and consumption as a superfood. Pharmacological studies show that the active constituents of the plant have effectively cured various diseases such as neuropathic pain, cancer, hypertension, diabetes, obesity etc. Nevertheless, several phytochemicals have yet to be explored for their possible therapeutic benefits. In addition to its clinical use, the plant is also used as an effective biostimulant for farmers in their fields and has proven to be a cost-effective alternative. A literature survey suggested that much preclinical research has been carried out in the last few years. In the future, more clinical studies are required to investigate the efficacy of the plant in life-threatening diseases such as coronavirus outbreaks, an acquired immunodeficiency syndrome (AIDS), and various cancers. Moreover, further mechanism base studies are also proposed to explore the mechanistic approach of the plant to identify and isolate active or synergistic compounds. Overall, M. oleifera signifies its name, “Miracle tree,” and appears to be a phytopharmaceutical and functional food that, if consumed daily, can potentially treat various chronic diseases in humans and could be used by medical practitioners as a safer alternative to treat various ailments.

Acknowledgments

The authors are grateful to Banasthali Vidhyapith for providing all the necessary resources for completing this report. The authors also thank Brijmohan Bairwa, Department of Remote Sensing, Banasthali Vidyapith, for technical support during the geospatial analysis.

Funding Statement

This research received no external funding.

Author Contributions

Conceptualization, A.P. (Ashutosh Pareek); methodology, A.P. (Aaushi Pareek) and M.P.; software, M.P.; writing—original draft preparation, M.P., P.K., V.J., M.M.G. and A.P. (Aaushi Pareek); validation, Y.R., A.A.C. and M.P.; writing—review and editing, A.P. (Ashutosh Pareek), A.A.C., Y.R. and M.M.G.; supervision, A.P. (Ashutosh Pareek); project administration, A.P. (Ashutosh Pareek) and A.A.C. All authors have read and agreed to the published version of the manuscript.

Institutional Review Board Statement

Informed consent statement, data availability statement, conflicts of interest.

The authors declare that they have no conflict of interest with respect to research, authorship, and/or publication of this article.

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