professional introduction thesis

How to write a fantastic thesis introduction (+15 examples)

The thesis introduction, usually chapter 1, is one of the most important chapters of a thesis. It sets the scene. It previews key arguments and findings. And it helps the reader to understand the structure of the thesis. In short, a lot is riding on this first chapter. With the following tips, you can write a powerful thesis introduction.

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Elements of a fantastic thesis introduction

Open with a (personal) story, begin with a problem, define a clear research gap, describe the scientific relevance of the thesis, describe the societal relevance of the thesis, write down the thesis’ core claim in 1-2 sentences, support your argument with sufficient evidence, consider possible objections, address the empirical research context, give a taste of the thesis’ empirical analysis, hint at the practical implications of the research, provide a reading guide, briefly summarise all chapters to come, design a figure illustrating the thesis structure.

An introductory chapter plays an integral part in every thesis. The first chapter has to include quite a lot of information to contextualise the research. At the same time, a good thesis introduction is not too long, but clear and to the point.

A powerful thesis introduction does the following:

• It captures the reader’s attention.
• It presents a clear research gap and emphasises the thesis’ relevance.
• It provides a compelling argument.
• It previews the research findings.
• It explains the structure of the thesis.

In addition, a powerful thesis introduction is well-written, logically structured, and free of grammar and spelling errors. Reputable thesis editors can elevate the quality of your introduction to the next level. If you are in search of a trustworthy thesis or dissertation editor who upholds high-quality standards and offers efficient turnaround times, I recommend the professional thesis and dissertation editing service provided by Editage . 

This list can feel quite overwhelming. However, with some easy tips and tricks, you can accomplish all these goals in your thesis introduction. (And if you struggle with finding the right wording, have a look at academic key phrases for introductions .)

Ways to capture the reader’s attention

A powerful thesis introduction should spark the reader’s interest on the first pages. A reader should be enticed to continue reading! There are three common ways to capture the reader’s attention.

An established way to capture the reader’s attention in a thesis introduction is by starting with a story. Regardless of how abstract and ‘scientific’ the actual thesis content is, it can be useful to ease the reader into the topic with a short story.

This story can be, for instance, based on one of your study participants. It can also be a very personal account of one of your own experiences, which drew you to study the thesis topic in the first place.

Start by providing data or statistics

Data and statistics are another established way to immediately draw in your reader. Especially surprising or shocking numbers can highlight the importance of a thesis topic in the first few sentences!

So if your thesis topic lends itself to being kick-started with data or statistics, you are in for a quick and easy way to write a memorable thesis introduction.

The third established way to capture the reader’s attention is by starting with the problem that underlies your thesis. It is advisable to keep the problem simple. A few sentences at the start of the chapter should suffice.

Usually, at a later stage in the introductory chapter, it is common to go more in-depth, describing the research problem (and its scientific and societal relevance) in more detail.

You may also like: Minimalist writing for a better thesis

Emphasising the thesis’ relevance

A good thesis is a relevant thesis. No one wants to read about a concept that has already been explored hundreds of times, or that no one cares about.

Of course, a thesis heavily relies on the work of other scholars. However, each thesis is – and should be – unique. If you want to write a fantastic thesis introduction, your job is to point out this uniqueness!

In academic research, a research gap signifies a research area or research question that has not been explored yet, that has been insufficiently explored, or whose insights and findings are outdated.

Every thesis needs a crystal-clear research gap. Spell it out instead of letting your reader figure out why your thesis is relevant.

* This example has been taken from an actual academic paper on toxic behaviour in online games: Liu, J. and Agur, C. (2022). “After All, They Don’t Know Me” Exploring the Psychological Mechanisms of Toxic Behavior in Online Games. Games and Culture 1–24, DOI: 10.1177/15554120221115397

The scientific relevance of a thesis highlights the importance of your work in terms of advancing theoretical insights on a topic. You can think of this part as your contribution to the (international) academic literature.

Scientific relevance comes in different forms. For instance, you can critically assess a prominent theory explaining a specific phenomenon. Maybe something is missing? Or you can develop a novel framework that combines different frameworks used by other scholars. Or you can draw attention to the context-specific nature of a phenomenon that is discussed in the international literature.

The societal relevance of a thesis highlights the importance of your research in more practical terms. You can think of this part as your contribution beyond theoretical insights and academic publications.

Why are your insights useful? Who can benefit from your insights? How can your insights improve existing practices?

Formulating a compelling argument

Arguments are sets of reasons supporting an idea, which – in academia – often integrate theoretical and empirical insights. Think of an argument as an umbrella statement, or core claim. It should be no longer than one or two sentences.

Including an argument in the introduction of your thesis may seem counterintuitive. After all, the reader will be introduced to your core claim before reading all the chapters of your thesis that led you to this claim in the first place.

But rest assured: A clear argument at the start of your thesis introduction is a sign of a good thesis. It works like a movie teaser to generate interest. And it helps the reader to follow your subsequent line of argumentation.

The core claim of your thesis should be accompanied by sufficient evidence. This does not mean that you have to write 10 pages about your results at this point.

However, you do need to show the reader that your claim is credible and legitimate because of the work you have done.

A good argument already anticipates possible objections. Not everyone will agree with your core claim. Therefore, it is smart to think ahead. What criticism can you expect?

Think about reasons or opposing positions that people can come up with to disagree with your claim. Then, try to address them head-on.

Providing a captivating preview of findings

Similar to presenting a compelling argument, a fantastic thesis introduction also previews some of the findings. When reading an introduction, the reader wants to learn a bit more about the research context. Furthermore, a reader should get a taste of the type of analysis that will be conducted. And lastly, a hint at the practical implications of the findings encourages the reader to read until the end.

If you focus on a specific empirical context, make sure to provide some information about it. The empirical context could be, for instance, a country, an island, a school or city. Make sure the reader understands why you chose this context for your research, and why it fits to your research objective.

If you did all your research in a lab, this section is obviously irrelevant. However, in that case you should explain the setup of your experiment, etcetera.

The empirical part of your thesis centers around the collection and analysis of information. What information, and what evidence, did you generate? And what are some of the key findings?

For instance, you can provide a short summary of the different research methods that you used to collect data. Followed by a short overview of how you analysed this data, and some of the key findings. The reader needs to understand why your empirical analysis is worth reading.

You already highlighted the practical relevance of your thesis in the introductory chapter. However, you should also provide a preview of some of the practical implications that you will develop in your thesis based on your findings.

Presenting a crystal clear thesis structure

A fantastic thesis introduction helps the reader to understand the structure and logic of your whole thesis. This is probably the easiest part to write in a thesis introduction. However, this part can be best written at the very end, once everything else is ready.

A reading guide is an essential part in a thesis introduction! Usually, the reading guide can be found toward the end of the introductory chapter.

The reading guide basically tells the reader what to expect in the chapters to come.

In a longer thesis, such as a PhD thesis, it can be smart to provide a summary of each chapter to come. Think of a paragraph for each chapter, almost in the form of an abstract.

For shorter theses, which also have a shorter introduction, this step is not necessary.

Especially for longer theses, it tends to be a good idea to design a simple figure that illustrates the structure of your thesis. It helps the reader to better grasp the logic of your thesis.

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How to write a good thesis introduction

1. Identify your readership

2. hook the reader and grab their attention, 3. provide relevant background, 4. give the reader a sense of what the paper is about, 5. preview key points and lead into your thesis statement, frequently asked questions about writing a good thesis introduction, related articles.

Many people struggle to write a thesis introduction. Much of your research prep should be done and you should be ready to start your introduction. But often, it’s not clear what needs to be included in a thesis introduction. If you feel stuck at this point not knowing how to start, this guide can help.

Tip: If you’re really struggling to write your thesis intro, consider putting in a placeholder until you write more of the body of your thesis. Then, come back to your intro once you have a stronger sense of the overall content of your thesis.

A good introduction draws readers in while providing the setup for the entire project. There is no single way to write an introduction that will always work for every topic , but the points below can act as a guide. These points can help you write a good thesis introduction.

Before even starting with your first sentence, consider who your readers are. Most likely, your readers will be the professors who are advising you on your thesis.

You should also consider readers of your thesis who are not specialists in your field. Writing with them in your mind will help you to be as clear as possible; this will make your thesis more understandable and enjoyable overall.

Tip: Always strive to be clear, correct, concrete, and concise in your writing.

The first sentence of the thesis is crucial. Looking back at your own research, think about how other writers may have hooked you.

It is common to start with a question or quotation, but these types of hooks are often overused. The best way to start your introduction is with a sentence that is broad and interesting and that seamlessly transitions into your argument.

Once again, consider your audience and how much background information they need to understand your approach. You can start by making a list of what is interesting about your topic:

• Are there any current events or controversies associated with your topic that might be interesting for your introduction?
• What kinds of background information might be useful for a reader to understand right away?
• Are there historical anecdotes or other situations that uniquely illustrate an important aspect of your argument?

A good introduction also needs to contain enough background information to allow the reader to understand the thesis statement and arguments. The amount of background information required will depend on the topic .

There should be enough background information so you don't have to spend too much time with it in the body of the thesis, but not so much that it becomes uninteresting.

Tip: Strike a balance between background information that is too broad or too specific.

Let the reader know what the purpose of the study is. Make sure to include the following points:

• Briefly describe the motivation behind your research.
• Describe the topic and scope of your research.
• Explain the practical relevance of your research.
• Explain the scholarly consensus related to your topic: briefly explain the most important articles and how they are related to your research.

At the end of your introduction, you should lead into your thesis statement by briefly bringing up a few of your main supporting details and by previewing what will be covered in the main part of the thesis. You’ll want to highlight the overall structure of your thesis so that readers will have a sense of what they will encounter as they read.

A good introduction draws readers in while providing the setup for the entire project. There is no single way to write an introduction that will always work for every topic, but these tips will help you write a great introduction:

• Identify your readership.
• Grab the reader's attention.
• Provide relevant background.
• Preview key points and lead into the thesis statement.

A good introduction needs to contain enough background information, and let the reader know what the purpose of the study is. Make sure to include the following points:

• Briefly describe the motivation for your research.

The length of the introduction will depend on the length of the whole thesis. Usually, an introduction makes up roughly 10 per cent of the total word count.

The best way to start your introduction is with a sentence that is broad and interesting and that seamlessly transitions into your argument. Consider the audience, then think of something that would grab their attention.

In Open Access: Theses and Dissertations you can find thousands of recent works. Take a look at any of the theses or dissertations for real-life examples of introductions that were already approved.

How To Write A Dissertation Introduction Chapter:

The 7 essential ingredients of an a-grade introduction.

By: Derek Jansen (MBA). Reviewed By Dr Eunice Rautenbach (D. Tech) | March 2020

If you’re reading this, you’re probably at the daunting early phases of writing up the introduction chapter of your dissertation or thesis. It can be intimidating, I know. 

In this post, we’ll look at the 7 essential ingredients of a strong dissertation or thesis introduction chapter, as well as the essential things you need to keep in mind as you craft each section. We’ll also share some useful tips to help you optimize your approach.

Overview: How To Write An Introduction Chapter

• Understand the purpose and function of the intro chapter
• Craft an enticing and engaging opening section
• Provide a background and context to the study
• Clearly define the research problem
• State your research aims, objectives and questions
• Explain the significance of your study
• Identify the limitations of your research
• Outline the structure of your dissertation or thesis

A quick sidenote:

You’ll notice that I’ve used the words dissertation and thesis interchangeably. While these terms reflect different levels of research – for example, Masters vs PhD-level research – the introduction chapter generally contains the same 7 essential ingredients regardless of level. So, in this post, dissertation introduction equals thesis introduction.

Start with why.

To craft a high-quality dissertation or thesis introduction chapter, you need to understand exactly what this chapter needs to achieve. In other words, what’s its purpose ? As the name suggests, the introduction chapter needs to introduce the reader to your research so that they understand what you’re trying to figure out, or what problem you’re trying to solve. More specifically, you need to answer four important questions in your introduction chapter.

These questions are:

• What will you be researching? (in other words, your research topic)
• Why is that worthwhile? (in other words, your justification)
• What will the scope of your research be? (in other words, what will you cover and what won’t you cover)
• What will the limitations of your research be? (in other words, what will the potential shortcomings of your research be?)

Simply put, your dissertation’s introduction chapter needs to provide an overview of your planned research , as well as a clear rationale for it. In other words, this chapter has to explain the “what” and the “why” of your research – what’s it all about and why’s that important.

Simple enough, right?

Well, the trick is finding the appropriate depth of information. As the researcher, you’ll be extremely close to your topic and this makes it easy to get caught up in the minor details. While these intricate details might be interesting, you need to write your introduction chapter on more of a “need-to-know” type basis, or it will end up way too lengthy and dense. You need to balance painting a clear picture with keeping things concise. Don’t worry though – you’ll be able to explore all the intricate details in later chapters.

Now that you understand what you need to achieve from your introduction chapter, we can get into the details. While the exact requirements for this chapter can vary from university to university, there are seven core components that most universities will require. We call these the seven essential ingredients . 

The 7 Essential Ingredients

• The opening section – where you’ll introduce the reader to your research in high-level terms
• The background to the study – where you’ll explain the context of your project
• The research problem – where you’ll explain the “gap” that exists in the current research
• The research aims , objectives and questions – where you’ll clearly state what your research will aim to achieve
• The significance (or justification) – where you’ll explain why your research is worth doing and the value it will provide to the world
• The limitations – where you’ll acknowledge the potential limitations of your project and approach
• The structure – where you’ll briefly outline the structure of your dissertation or thesis to help orient the reader

By incorporating these seven essential ingredients into your introduction chapter, you’ll comprehensively cover both the “ what ” and the “ why ” I mentioned earlier – in other words, you’ll achieve the purpose of the chapter.

Side note – you can also use these 7 ingredients in this order as the structure for your chapter to ensure a smooth, logical flow. This isn’t essential, but, generally speaking, it helps create an engaging narrative that’s easy for your reader to understand. If you’d like, you can also download our free introduction chapter template here.

Alright – let’s look at each of the ingredients now.

#1 – The Opening Section

The very first essential ingredient for your dissertation introduction is, well, an introduction or opening section. Just like every other chapter, your introduction chapter needs to start by providing a brief overview of what you’ll be covering in the chapter.

This section needs to engage the reader with clear, concise language that can be easily understood and digested. If the reader (your marker!) has to struggle through it, they’ll lose interest, which will make it harder for you to earn marks. Just because you’re writing an academic paper doesn’t mean you can ignore the basic principles of engaging writing used by marketers, bloggers, and journalists. At the end of the day, you’re all trying to sell an idea – yours is just a research idea.

So, what goes into this opening section?

Well, while there’s no set formula, it’s a good idea to include the following four foundational sentences in your opening section:

1 – A sentence or two introducing the overall field of your research.

For example:

“Organisational skills development involves identifying current or potential skills gaps within a business and developing programs to resolve these gaps. Management research, including X, Y and Z, has clearly established that organisational skills development is an essential contributor to business growth.”

2 – A sentence introducing your specific research problem.

“However, there are conflicting views and an overall lack of research regarding how best to manage skills development initiatives in highly dynamic environments where subject knowledge is rapidly and continuously evolving – for example, in the website development industry.”

3 – A sentence stating your research aims and objectives.

“This research aims to identify and evaluate skills development approaches and strategies for highly dynamic industries in which subject knowledge is continuously evolving.”.

4 – A sentence outlining the layout of the chapter.

“This chapter will provide an introduction to the study by first discussing the background and context, followed by the research problem, the research aims, objectives and questions, the significance and finally, the limitations.”

As I mentioned, this opening section of your introduction chapter shouldn’t be lengthy . Typically, these four sentences should fit neatly into one or two paragraphs, max. What you’re aiming for here is a clear, concise introduction to your research – not a detailed account.

PS – If some of this terminology sounds unfamiliar, don’t stress – I’ll explain each of the concepts later in this post.

#2 – Background to the study

Now that you’ve provided a high-level overview of your dissertation or thesis, it’s time to go a little deeper and lay a foundation for your research topic. This foundation is what the second ingredient is all about – the background to your study.

So, what is the background section all about?

Well, this section of your introduction chapter should provide a broad overview of the topic area that you’ll be researching, as well as the current contextual factors . This could include, for example, a brief history of the topic, recent developments in the area, key pieces of research in the area and so on. In other words, in this section, you need to provide the relevant background information to give the reader a decent foundational understanding of your research area.

Let’s look at an example to make this a little more concrete.

If we stick with the skills development topic I mentioned earlier, the background to the study section would start by providing an overview of the skills development area and outline the key existing research. Then, it would go on to discuss how the modern-day context has created a new challenge for traditional skills development strategies and approaches. Specifically, that in many industries, technical knowledge is constantly and rapidly evolving, and traditional education providers struggle to keep up with the pace of new technologies.

Importantly, you need to write this section with the assumption that the reader is not an expert in your topic area. So, if there are industry-specific jargon and complex terminology, you should briefly explain that here , so that the reader can understand the rest of your document.

Don’t make assumptions about the reader’s knowledge – in most cases, your markers will not be able to ask you questions if they don’t understand something. So, always err on the safe side and explain anything that’s not common knowledge.

#3 – The research problem

Now that you’ve given your reader an overview of your research area, it’s time to get specific about the research problem that you’ll address in your dissertation or thesis. While the background section would have alluded to a potential research problem (or even multiple research problems), the purpose of this section is to narrow the focus and highlight the specific research problem you’ll focus on.

But, what exactly is a research problem, you ask?

Well, a research problem can be any issue or question for which there isn’t already a well-established and agreed-upon answer in the existing research. In other words, a research problem exists when there’s a need to answer a question (or set of questions), but there’s a gap in the existing literature , or the existing research is conflicting and/or inconsistent.

So, to present your research problem, you need to make it clear what exactly is missing in the current literature and why this is a problem . It’s usually a good idea to structure this discussion into three sections – specifically:

• What’s already well-established in the literature (in other words, the current state of research)
• What’s missing in the literature (in other words, the literature gap)
• Why this is a problem (in other words, why it’s important to fill this gap)

Let’s look at an example of this structure using the skills development topic.

Organisational skills development is critically important for employee satisfaction and company performance (reference). Numerous studies have investigated strategies and approaches to manage skills development programs within organisations (reference).

(this paragraph explains what’s already well-established in the literature)

However, these studies have traditionally focused on relatively slow-paced industries where key skills and knowledge do not change particularly often. This body of theory presents a problem for industries that face a rapidly changing skills landscape – for example, the website development industry – where new platforms, languages and best practices emerge on an extremely frequent basis.

(this paragraph explains what’s missing from the literature)

As a result, the existing research is inadequate for industries in which essential knowledge and skills are constantly and rapidly evolving, as it assumes a slow pace of knowledge development. Industries in such environments, therefore, find themselves ill-equipped in terms of skills development strategies and approaches.

(this paragraph explains why the research gap is problematic)

As you can see in this example, in a few lines, we’ve explained (1) the current state of research, (2) the literature gap and (3) why that gap is problematic. By doing this, the research problem is made crystal clear, which lays the foundation for the next ingredient.

#4 – The research aims, objectives and questions

Now that you’ve clearly identified your research problem, it’s time to identify your research aims and objectives , as well as your research questions . In other words, it’s time to explain what you’re going to do about the research problem.

So, what do you need to do here?

Well, the starting point is to clearly state your research aim (or aims) . The research aim is the main goal or the overarching purpose of your dissertation or thesis. In other words, it’s a high-level statement of what you’re aiming to achieve.

Let’s look at an example, sticking with the skills development topic:

“Given the lack of research regarding organisational skills development in fast-moving industries, this study will aim to identify and evaluate the skills development approaches utilised by web development companies in the UK”.

As you can see in this example, the research aim is clearly outlined, as well as the specific context in which the research will be undertaken (in other words, web development companies in the UK).

Next up is the research objective (or objectives) . While the research aims cover the high-level “what”, the research objectives are a bit more practically oriented, looking at specific things you’ll be doing to achieve those research aims.

Let’s take a look at an example of some research objectives (ROs) to fit the research aim.

• RO1 – To identify common skills development strategies and approaches utilised by web development companies in the UK.
• RO2 – To evaluate the effectiveness of these strategies and approaches.
• RO3 – To compare and contrast these strategies and approaches in terms of their strengths and weaknesses.

As you can see from this example, these objectives describe the actions you’ll take and the specific things you’ll investigate in order to achieve your research aims. They break down the research aims into more specific, actionable objectives.

The final step is to state your research questions . Your research questions bring the aims and objectives another level “down to earth”. These are the specific questions that your dissertation or theses will seek to answer. They’re not fluffy, ambiguous or conceptual – they’re very specific and you’ll need to directly answer them in your conclusions chapter .

The research questions typically relate directly to the research objectives and sometimes can look a bit obvious, but they are still extremely important. Let’s take a look at an example of the research questions (RQs) that would flow from the research objectives I mentioned earlier.

• RQ1 – What skills development strategies and approaches are currently being used by web development companies in the UK?
• RQ2 – How effective are each of these strategies and approaches?
• RQ3 – What are the strengths and weaknesses of each of these strategies and approaches?

As you can see, the research questions mimic the research objectives , but they are presented in question format. These questions will act as the driving force throughout your dissertation or thesis – from the literature review to the methodology and onward – so they’re really important.

A final note about this section – it’s really important to be clear about the scope of your study (more technically, the delimitations ). In other words, what you WILL cover and what you WON’T cover. If your research aims, objectives and questions are too broad, you’ll risk losing focus or investigating a problem that is too big to solve within a single dissertation.

Simply put, you need to establish clear boundaries in your research. You can do this, for example, by limiting it to a specific industry, country or time period. That way, you’ll ringfence your research, which will allow you to investigate your topic deeply and thoroughly – which is what earns marks!

Need a helping hand?

#5 – Significance

Now that you’ve made it clear what you’ll be researching, it’s time to make a strong argument regarding your study’s importance and significance . In other words, now that you’ve covered the what, it’s time to cover the why – enter essential ingredient number 5 – significance.

Of course, by this stage, you’ve already briefly alluded to the importance of your study in your background and research problem sections, but you haven’t explicitly stated how your research findings will benefit the world . So, now’s your chance to clearly state how your study will benefit either industry , academia , or – ideally – both . In other words, you need to explain how your research will make a difference and what implications it will have.

Let’s take a look at an example.

“This study will contribute to the body of knowledge on skills development by incorporating skills development strategies and approaches for industries in which knowledge and skills are rapidly and constantly changing. This will help address the current shortage of research in this area and provide real-world value to organisations operating in such dynamic environments.”

As you can see in this example, the paragraph clearly explains how the research will help fill a gap in the literature and also provide practical real-world value to organisations.

This section doesn’t need to be particularly lengthy, but it does need to be convincing . You need to “sell” the value of your research here so that the reader understands why it’s worth committing an entire dissertation or thesis to it. This section needs to be the salesman of your research. So, spend some time thinking about the ways in which your research will make a unique contribution to the world and how the knowledge you create could benefit both academia and industry – and then “sell it” in this section.

#6 – The limitations

Now that you’ve “sold” your research to the reader and hopefully got them excited about what’s coming up in the rest of your dissertation, it’s time to briefly discuss the potential limitations of your research.

But you’re probably thinking, hold up – what limitations? My research is well thought out and carefully designed – why would there be limitations?

Well, no piece of research is perfect . This is especially true for a dissertation or thesis – which typically has a very low or zero budget, tight time constraints and limited researcher experience. Generally, your dissertation will be the first or second formal research project you’ve ever undertaken, so it’s unlikely to win any research awards…

Simply put, your research will invariably have limitations. Don’t stress yourself out though – this is completely acceptable (and expected). Even “professional” research has limitations – as I said, no piece of research is perfect. The key is to recognise the limitations upfront and be completely transparent about them, so that future researchers are aware of them and can improve the study’s design to minimise the limitations and strengthen the findings.

Generally, you’ll want to consider at least the following four common limitations. These are:

• Your scope – for example, perhaps your focus is very narrow and doesn’t consider how certain variables interact with each other.
• Your research methodology – for example, a qualitative methodology could be criticised for being overly subjective, or a quantitative methodology could be criticised for oversimplifying the situation (learn more about methodologies here ).
• Your resources – for example, a lack of time, money, equipment and your own research experience.
• The generalisability of your findings – for example, the findings from the study of a specific industry or country can’t necessarily be generalised to other industries or countries.

Don’t be shy here. There’s no use trying to hide the limitations or weaknesses of your research. In fact, the more critical you can be of your study, the better. The markers want to see that you are aware of the limitations as this demonstrates your understanding of research design – so be brutal.

#7 – The structural outline

Now that you’ve clearly communicated what your research is going to be about, why it’s important and what the limitations of your research will be, the final ingredient is the structural outline.The purpose of this section is simply to provide your reader with a roadmap of what to expect in terms of the structure of your dissertation or thesis.

In this section, you’ll need to provide a brief summary of each chapter’s purpose and contents (including the introduction chapter). A sentence or two explaining what you’ll do in each chapter is generally enough to orient the reader. You don’t want to get too detailed here – it’s purely an outline, not a summary of your research.

Let’s look at an example:

In Chapter One, the context of the study has been introduced. The research objectives and questions have been identified, and the value of such research argued. The limitations of the study have also been discussed.

In Chapter Two, the existing literature will be reviewed and a foundation of theory will be laid out to identify key skills development approaches and strategies within the context of fast-moving industries, especially technology-intensive industries.

In Chapter Three, the methodological choices will be explored. Specifically, the adoption of a qualitative, inductive research approach will be justified, and the broader research design will be discussed, including the limitations thereof.

So, as you can see from the example, this section is simply an outline of the chapter structure, allocating a short paragraph to each chapter. Done correctly, the outline will help your reader understand what to expect and reassure them that you’ll address the multiple facets of the study.

By the way – if you’re unsure of how to structure your dissertation or thesis, be sure to check out our video post which explains dissertation structure .

Keep calm and carry on.

Hopefully you feel a bit more prepared for this challenge of crafting your dissertation or thesis introduction chapter now. Take a deep breath and remember that Rome wasn’t built in a day – conquer one ingredient at a time and you’ll be firmly on the path to success.

Let’s quickly recap – the 7 ingredients are:

• The opening section – where you give a brief, high-level overview of what your research will be about.
• The study background – where you introduce the reader to key theory, concepts and terminology, as well as the context of your study.
• The research problem – where you explain what the problem with the current research is. In other words, the research gap.
• The research aims , objectives and questions – where you clearly state what your dissertation will investigate.
• The significance – where you explain what value your research will provide to the world.
• The limitations – where you explain what the potential shortcomings and limitations of your research may be.
• The structural outline – where you provide a high-level overview of the structure of your document

If you bake these ingredients into your dissertation introduction chapter, you’ll be well on your way to building an engaging introduction chapter that lays a rock-solid foundation for the rest of your document.

Remember, while we’ve covered the essential ingredients here, there may be some additional components that your university requires, so be sure to double-check your project brief!

Psst... there’s more!

This post was based on one of our popular Research Bootcamps . If you're working on a research project, you'll definitely want to check this out ...

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Thanks very much for such an insight. I feel confident enough in undertaking my thesis on the survey;The future of facial recognition and learning non verbal interaction

Glad to hear that. Good luck with your thesis!

Thanks very much for such an insight. I feel confident now undertaking my thesis; The future of facial recognition and learning non verbal interaction.

Thanks so much for this article. I found myself struggling and wasting a lot of time in my thesis writing but after reading this article and watching some of your youtube videos, I now have a clear understanding of what is required for a thesis.

Thank you Derek, i find your each post so useful. Keep it up.

Thank you so much Derek ,for shedding the light and making it easier for me to handle the daunting task of academic writing .

Thanks do much Dereck for the comprehensive guide. It will assist me queit a lot in my thesis.

thanks a lot for helping

i LOVE the gifs, such a fun way to engage readers. thanks for the advice, much appreciated

Thanks a lot Derek! It will be really useful to the beginner in research!

You’re welcome

This is a well written, easily comprehensible, simple introduction to the basics of a Research Dissertation../the need to keep the reader in mind while writing the dissertation is an important point that is covered../ I appreciate the efforts of the author../

The instruction given are perfect and clear. I was supposed to take the course , unfortunately in Nepal the service is not avaialble.However, I am much more hopeful that you will provide require documents whatever you have produced so far.

Thank you very much

Thanks so much ❤️😘 I feel am ready to start writing my research methodology

This is genuinely the most effective advice I have ever been given regarding academia. Thank you so much!

This is one of the best write up I have seen in my road to PhD thesis. regards, this write up update my knowledge of research

I was looking for some good blogs related to Education hopefully your article will help. Thanks for sharing.

This is an awesome masterpiece. It is one of the most comprehensive guides to writing a Dissertation/Thesis I have seen and read.

You just saved me from going astray in writing a Dissertation for my undergraduate studies. I could not be more grateful for such a relevant guide like this. Thank you so much.

Thank you so much Derek, this has been extremely helpful!!

I do have one question though, in the limitations part do you refer to the scope as the focus of the research on a specific industry/country/chronological period? I assume that in order to talk about whether or not the research could be generalized, the above would need to be already presented and described in the introduction.

Thank you again!

Phew! You have genuinely rescued me. I was stuck how to go about my thesis. Now l have started. Thank you.

This is the very best guide in anything that has to do with thesis or dissertation writing. The numerous blends of examples and detailed insights make it worth a read and in fact, a treasure that is worthy to be bookmarked.

Thanks a lot for this masterpiece!

Powerful insight. I can now take a step

Thank you very much for these valuable introductions to thesis chapters. I saw all your videos about writing the introduction, discussion, and conclusion chapter. Then, I am wondering if we need to explain our research limitations in all three chapters, introduction, discussion, and conclusion? Isn’t it a bit redundant? If not, could you please explain how can we write in different ways? Thank you.

Excellent!!! Thank you…

Thanks for this informative content. I have a question. The research gap is mentioned in both the introduction and literature section. I would like to know how can I demonstrate the research gap in both sections without repeating the contents?

I’m incredibly grateful for this invaluable content. I’ve been dreading compiling my postgrad thesis but breaking each chapter down into sections has made it so much easier for me to engage with the material without feeling overwhelmed. After relying on your guidance, I’m really happy with how I’ve laid out my introduction.

Thank you for the informative content you provided

Hi Derrick and Team, thank you so much for the comprehensive guide on how to write a dissertation or a thesis introduction section. For some of us first-timers, it is a daunting task. However, the instruction with relevant examples makes it clear and easy to follow through. Much appreciated.

It was so helpful. God Bless you. Thanks very much

I thank you Grad coach for your priceless help. I have two questions I have learned from your video the limitations of the research presented in chapter one. but in another video also presented in chapter five. which chapter limitation should be included? If possible, I need your answer since I am doing my thesis. how can I explain If I am asked what is my motivation for this research?

Thank you guys for the great work you are doing. Honestly, you have made the research to be interesting and simplified. Even a novice will easily grasp the ideas you put forward, Thank you once again.

Excellent piece!

I feel like just settling for a good topic is usually the hardest part.

Thank you so much. My confidence has been completely destroyed during my first year of PhD and you have helped me pull myself together again

Happy to help 🙂

I am so glad I ran into your resources and did not waste time doing the wrong this. Research is now making so much sense now.

Gratitude to Derrick and the team I was looking for a solid article that would aid me in drafting the thesis’ introduction. I felt quite happy when I came across the piece you wrote because it was so well-written and insightful. I wish you success in the future.

thank you so much. God Bless you

Thank you so much Grad Coach for these helpful insights. Now I can get started, with a great deal of confidence.

It’s ‘alluded to’ not ‘eluded to’.

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How to Write a Thesis Introduction

What types of information should you include in your introduction .

In the introduction of your thesis, you’ll be trying to do three main things, which are called Moves :

• Move 1 establish your territory (say what the topic is about)
• Move 2 establish a niche (show why there needs to be further research on your topic)
• Move 3 introduce the current research (make hypotheses; state the research questions)

Each Move has a number of stages. Depending on what you need to say in your introduction, you might use one or more stages. Table 1 provides you with a list of the most commonly occurring stages of introductions in Honours theses (colour-coded to show the Moves ). You will also find examples of Introductions, divided into stages with sample sentence extracts. Once you’ve looked at Examples 1 and 2, try the exercise that follows.

Most thesis introductions include SOME (but not all) of the stages listed below. There are variations between different Schools and between different theses, depending on the purpose of the thesis.

Stages in a thesis introduction

• state the general topic and give some background
• provide a review of the literature related to the topic
• define the terms and scope of the topic
• outline the current situation
• evaluate the current situation (advantages/ disadvantages) and identify the gap
• identify the importance of the proposed research
• state the research problem/ questions
• state the research aims and/or research objectives
• state the hypotheses
• outline the order of information in the thesis
• outline the methodology

Example 1: Evaluation of Boron Solid Source Diffusion for High-Efficiency Silicon Solar Cells (School of Photovoltaic and Renewable Energy Engineering)

Example 2: Methods for Measuring Hepatitis C Viral Complexity (School of Biotechnology and Biological Sciences)

Note: this introduction includes the literature review.

Now that you have read example 1 and 2, what are the differences?

Example 3: The IMO Severe-Weather Criterion Applied to High-Speed Monohulls (School of Mechanical and Manufacturing Engineering)

Example 4: The Steiner Tree Problem (School of Computer Science and Engineering)

Introduction exercise

Example 5.1 (extract 1): The effects of Fluoride on the reproduction of three native Australian plant Species (School of Geography)

Example 5.2 (extract 2): The effects of Fluoride on the reproduction of three native Australian plant Species (School of Geography)

Example 5.3

Example 5.4 (extract 4): The effects of Fluoride on the reproduction of three native Australian plant Species (School of Geography)

Example 5.5 (extract 5): The effects of Fluoride on the reproduction of three native Australian plant Species (School of Geography)

Example 5.6 (extract 6): The effects of Fluoride on the reproduction of three native Australian plant Species (School of Geography)

Well, firstly, there are many choices that you can make. You will notice that there are variations not only between the different Schools in your faculty, but also between individual theses, depending on the type of information that is being communicated. However, there are a few elements that a good Introduction should include, at the very minimum:

• Either Statement of general topic Or Background information about the topic;
• Either Identification of disadvantages of current situation Or Identification of the gap in current research;
• Identification of importance of proposed research
• Either Statement of aims Or Statement of objectives
• An Outline of the order of information in the thesis

Engineering & science

• Report writing
• Technical writing
• Writing lab reports
• Introductions
• Literature review
• Writing up results
• Discussions
• Conclusions
• Writing tools
• Case study report in (engineering)
• ^ More support

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• If you are writing in a new discipline, you should always make sure to ask about conventions and expectations for introductions, just as you would for any other aspect of the essay. For example, while it may be acceptable to write a two-paragraph (or longer) introduction for your papers in some courses, instructors in other disciplines, such as those in some Government courses, may expect a shorter introduction that includes a preview of the argument that will follow.  
• In some disciplines (Government, Economics, and others), it’s common to offer an overview in the introduction of what points you will make in your essay. In other disciplines, you will not be expected to provide this overview in your introduction.  
• Avoid writing a very general opening sentence. While it may be true that “Since the dawn of time, people have been telling love stories,” it won’t help you explain what’s interesting about your topic.  
• Avoid writing a “funnel” introduction in which you begin with a very broad statement about a topic and move to a narrow statement about that topic. Broad generalizations about a topic will not add to your readers’ understanding of your specific essay topic.  
• Avoid beginning with a dictionary definition of a term or concept you will be writing about. If the concept is complicated or unfamiliar to your readers, you will need to define it in detail later in your essay. If it’s not complicated, you can assume your readers already know the definition.  
• Avoid offering too much detail in your introduction that a reader could better understand later in the paper.
• picture_as_pdf Introductions

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How to Write a Research Paper Introduction (with Examples)

The research paper introduction section, along with the Title and Abstract, can be considered the face of any research paper. The following article is intended to guide you in organizing and writing the research paper introduction for a quality academic article or dissertation.

The research paper introduction aims to present the topic to the reader. A study will only be accepted for publishing if you can ascertain that the available literature cannot answer your research question. So it is important to ensure that you have read important studies on that particular topic, especially those within the last five to ten years, and that they are properly referenced in this section. 1 What should be included in the research paper introduction is decided by what you want to tell readers about the reason behind the research and how you plan to fill the knowledge gap. The best research paper introduction provides a systemic review of existing work and demonstrates additional work that needs to be done. It needs to be brief, captivating, and well-referenced; a well-drafted research paper introduction will help the researcher win half the battle.

The introduction for a research paper is where you set up your topic and approach for the reader. It has several key goals:

• Present your research topic
• Capture reader interest
• Summarize existing research
• Position your own approach
• Define your specific research problem and problem statement
• Highlight the novelty and contributions of the study
• Give an overview of the paper’s structure

The research paper introduction can vary in size and structure depending on whether your paper presents the results of original empirical research or is a review paper. Some research paper introduction examples are only half a page while others are a few pages long. In many cases, the introduction will be shorter than all of the other sections of your paper; its length depends on the size of your paper as a whole.

• Break through writer’s block. Write your research paper introduction with Paperpal Copilot

Table of Contents

What is the introduction for a research paper, why is the introduction important in a research paper, craft a compelling introduction section with paperpal. try now, 1. introduce the research topic:, 2. determine a research niche:, 3. place your research within the research niche:, craft accurate research paper introductions with paperpal. start writing now, frequently asked questions on research paper introduction, key points to remember.

The introduction in a research paper is placed at the beginning to guide the reader from a broad subject area to the specific topic that your research addresses. They present the following information to the reader

• Scope: The topic covered in the research paper
• Context: Background of your topic
• Importance: Why your research matters in that particular area of research and the industry problem that can be targeted

The research paper introduction conveys a lot of information and can be considered an essential roadmap for the rest of your paper. A good introduction for a research paper is important for the following reasons:

• It stimulates your reader’s interest: A good introduction section can make your readers want to read your paper by capturing their interest. It informs the reader what they are going to learn and helps determine if the topic is of interest to them.
• It helps the reader understand the research background: Without a clear introduction, your readers may feel confused and even struggle when reading your paper. A good research paper introduction will prepare them for the in-depth research to come. It provides you the opportunity to engage with the readers and demonstrate your knowledge and authority on the specific topic.
• It explains why your research paper is worth reading: Your introduction can convey a lot of information to your readers. It introduces the topic, why the topic is important, and how you plan to proceed with your research.
• It helps guide the reader through the rest of the paper: The research paper introduction gives the reader a sense of the nature of the information that will support your arguments and the general organization of the paragraphs that will follow. It offers an overview of what to expect when reading the main body of your paper.

What are the parts of introduction in the research?

A good research paper introduction section should comprise three main elements: 2

• What is known: This sets the stage for your research. It informs the readers of what is known on the subject.
• What is lacking: This is aimed at justifying the reason for carrying out your research. This could involve investigating a new concept or method or building upon previous research.
• What you aim to do: This part briefly states the objectives of your research and its major contributions. Your detailed hypothesis will also form a part of this section.

How to write a research paper introduction?

The first step in writing the research paper introduction is to inform the reader what your topic is and why it’s interesting or important. This is generally accomplished with a strong opening statement. The second step involves establishing the kinds of research that have been done and ending with limitations or gaps in the research that you intend to address. Finally, the research paper introduction clarifies how your own research fits in and what problem it addresses. If your research involved testing hypotheses, these should be stated along with your research question. The hypothesis should be presented in the past tense since it will have been tested by the time you are writing the research paper introduction.

The following key points, with examples, can guide you when writing the research paper introduction section:

• Highlight the importance of the research field or topic
• Describe the background of the topic
• Present an overview of current research on the topic

Example: The inclusion of experiential and competency-based learning has benefitted electronics engineering education. Industry partnerships provide an excellent alternative for students wanting to engage in solving real-world challenges. Industry-academia participation has grown in recent years due to the need for skilled engineers with practical training and specialized expertise. However, from the educational perspective, many activities are needed to incorporate sustainable development goals into the university curricula and consolidate learning innovation in universities.

• Reveal a gap in existing research or oppose an existing assumption
• Formulate the research question

Example: There have been plausible efforts to integrate educational activities in higher education electronics engineering programs. However, very few studies have considered using educational research methods for performance evaluation of competency-based higher engineering education, with a focus on technical and or transversal skills. To remedy the current need for evaluating competencies in STEM fields and providing sustainable development goals in engineering education, in this study, a comparison was drawn between study groups without and with industry partners.

• State the purpose of your study
• Highlight the key characteristics of your study
• Describe important results
• Highlight the novelty of the study.
• Offer a brief overview of the structure of the paper.

Example: The study evaluates the main competency needed in the applied electronics course, which is a fundamental core subject for many electronics engineering undergraduate programs. We compared two groups, without and with an industrial partner, that offered real-world projects to solve during the semester. This comparison can help determine significant differences in both groups in terms of developing subject competency and achieving sustainable development goals.

Write a Research Paper Introduction in Minutes with Paperpal

Paperpal Copilot is a generative AI-powered academic writing assistant. It’s trained on millions of published scholarly articles and over 20 years of STM experience. Paperpal Copilot helps authors write better and faster with:

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With Paperpal Copilot, create a research paper introduction effortlessly. In this step-by-step guide, we’ll walk you through how Paperpal transforms your initial ideas into a polished and publication-ready introduction.

How to use Paperpal to write the Introduction section

Step 1: Sign up on Paperpal and click on the Copilot feature, under this choose Outlines > Research Article > Introduction

Step 2: Add your unstructured notes or initial draft, whether in English or another language, to Paperpal, which is to be used as the base for your content.

Step 3: Fill in the specifics, such as your field of study, brief description or details you want to include, which will help the AI generate the outline for your Introduction.

Step 4: Use this outline and sentence suggestions to develop your content, adding citations where needed and modifying it to align with your specific research focus.

Step 5: Turn to Paperpal’s granular language checks to refine your content, tailor it to reflect your personal writing style, and ensure it effectively conveys your message.

You can use the same process to develop each section of your article, and finally your research paper in half the time and without any of the stress.

The purpose of the research paper introduction is to introduce the reader to the problem definition, justify the need for the study, and describe the main theme of the study. The aim is to gain the reader’s attention by providing them with necessary background information and establishing the main purpose and direction of the research.

The length of the research paper introduction can vary across journals and disciplines. While there are no strict word limits for writing the research paper introduction, an ideal length would be one page, with a maximum of 400 words over 1-4 paragraphs. Generally, it is one of the shorter sections of the paper as the reader is assumed to have at least a reasonable knowledge about the topic. 2 For example, for a study evaluating the role of building design in ensuring fire safety, there is no need to discuss definitions and nature of fire in the introduction; you could start by commenting upon the existing practices for fire safety and how your study will add to the existing knowledge and practice.

When deciding what to include in the research paper introduction, the rest of the paper should also be considered. The aim is to introduce the reader smoothly to the topic and facilitate an easy read without much dependency on external sources. 3 Below is a list of elements you can include to prepare a research paper introduction outline and follow it when you are writing the research paper introduction. Topic introduction: This can include key definitions and a brief history of the topic. Research context and background: Offer the readers some general information and then narrow it down to specific aspects. Details of the research you conducted: A brief literature review can be included to support your arguments or line of thought. Rationale for the study: This establishes the relevance of your study and establishes its importance. Importance of your research: The main contributions are highlighted to help establish the novelty of your study Research hypothesis: Introduce your research question and propose an expected outcome. Organization of the paper: Include a short paragraph of 3-4 sentences that highlights your plan for the entire paper

Cite only works that are most relevant to your topic; as a general rule, you can include one to three. Note that readers want to see evidence of original thinking. So it is better to avoid using too many references as it does not leave much room for your personal standpoint to shine through. Citations in your research paper introduction support the key points, and the number of citations depend on the subject matter and the point discussed. If the research paper introduction is too long or overflowing with citations, it is better to cite a few review articles rather than the individual articles summarized in the review. A good point to remember when citing research papers in the introduction section is to include at least one-third of the references in the introduction.

The literature review plays a significant role in the research paper introduction section. A good literature review accomplishes the following: Introduces the topic – Establishes the study’s significance – Provides an overview of the relevant literature – Provides context for the study using literature – Identifies knowledge gaps However, remember to avoid making the following mistakes when writing a research paper introduction: Do not use studies from the literature review to aggressively support your research Avoid direct quoting Do not allow literature review to be the focus of this section. Instead, the literature review should only aid in setting a foundation for the manuscript.

Remember the following key points for writing a good research paper introduction: 4

• Avoid stuffing too much general information: Avoid including what an average reader would know and include only that information related to the problem being addressed in the research paper introduction. For example, when describing a comparative study of non-traditional methods for mechanical design optimization, information related to the traditional methods and differences between traditional and non-traditional methods would not be relevant. In this case, the introduction for the research paper should begin with the state-of-the-art non-traditional methods and methods to evaluate the efficiency of newly developed algorithms.
• Avoid packing too many references: Cite only the required works in your research paper introduction. The other works can be included in the discussion section to strengthen your findings.
• Avoid extensive criticism of previous studies: Avoid being overly critical of earlier studies while setting the rationale for your study. A better place for this would be the Discussion section, where you can highlight the advantages of your method.
• Avoid describing conclusions of the study: When writing a research paper introduction remember not to include the findings of your study. The aim is to let the readers know what question is being answered. The actual answer should only be given in the Results and Discussion section.

To summarize, the research paper introduction section should be brief yet informative. It should convince the reader the need to conduct the study and motivate him to read further. If you’re feeling stuck or unsure, choose trusted AI academic writing assistants like Paperpal to effortlessly craft your research paper introduction and other sections of your research article.

1. Jawaid, S. A., & Jawaid, M. (2019). How to write introduction and discussion. Saudi Journal of Anaesthesia, 13(Suppl 1), S18.

2. Dewan, P., & Gupta, P. (2016). Writing the title, abstract and introduction: Looks matter!. Indian pediatrics, 53, 235-241.

3. Cetin, S., & Hackam, D. J. (2005). An approach to the writing of a scientific Manuscript1. Journal of Surgical Research, 128(2), 165-167.

4. Bavdekar, S. B. (2015). Writing introduction: Laying the foundations of a research paper. Journal of the Association of Physicians of India, 63(7), 44-6.

Paperpal is a comprehensive AI writing toolkit that helps students and researchers achieve 2x the writing in half the time. It leverages 21+ years of STM experience and insights from millions of research articles to provide in-depth academic writing, language editing, and submission readiness support to help you write better, faster.  

Get accurate academic translations, rewriting support, grammar checks, vocabulary suggestions, and generative AI assistance that delivers human precision at machine speed. Try for free or upgrade to Paperpal Prime starting at US$19 a month to access premium features, including consistency, plagiarism, and 30+ submission readiness checks to help you succeed.  

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• 8 Most Effective Ways to Increase Motivation for Thesis Writing 

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• Dissertation

How to Write a Thesis or Dissertation Introduction

Published on 9 September 2022 by Tegan George and Shona McCombes.

The introduction is the first section of your thesis or dissertation , appearing right after the table of contents . Your introduction draws your reader in, setting the stage for your research with a clear focus, purpose, and direction.

Your introduction should include:

• Your topic, in context: what does your reader need to know to understand your thesis dissertation?
• Your focus and scope: what specific aspect of the topic will you address?
• The relevance of your research: how does your work fit into existing studies on your topic?
• Your questions and objectives: what does your research aim to find out, and how?
• An overview of your structure: what does each section contribute to the overall aim?

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Table of contents

How to start your introduction, topic and context, focus and scope, relevance and importance, questions and objectives, overview of the structure, thesis introduction example, introduction checklist, frequently asked questions about introductions.

Although your introduction kicks off your dissertation, it doesn’t have to be the first thing you write – in fact, it’s often one of the very last parts to be completed (just before your abstract ).

It’s a good idea to write a rough draft of your introduction as you begin your research, to help guide you. If you wrote a research proposal , consider using this as a template, as it contains many of the same elements. However, be sure to revise your introduction throughout the writing process, making sure it matches the content of your ensuing sections.

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Begin by introducing your research topic and giving any necessary background information. It’s important to contextualise your research and generate interest. Aim to show why your topic is timely or important. You may want to mention a relevant news item, academic debate, or practical problem.

After a brief introduction to your general area of interest, narrow your focus and define the scope of your research.

You can narrow this down in many ways, such as by:

• Geographical area
• Time period
• Demographics or communities
• Themes or aspects of the topic

It’s essential to share your motivation for doing this research, as well as how it relates to existing work on your topic. Further, you should also mention what new insights you expect it will contribute.

Start by giving a brief overview of the current state of research. You should definitely cite the most relevant literature, but remember that you will conduct a more in-depth survey of relevant sources in the literature review section, so there’s no need to go too in-depth in the introduction.

Depending on your field, the importance of your research might focus on its practical application (e.g., in policy or management) or on advancing scholarly understanding of the topic (e.g., by developing theories or adding new empirical data). In many cases, it will do both.

Ultimately, your introduction should explain how your thesis or dissertation:

• Helps solve a practical or theoretical problem
• Addresses a gap in the literature
• Builds on existing research
• Proposes a new understanding of your topic

Perhaps the most important part of your introduction is your questions and objectives, as it sets up the expectations for the rest of your thesis or dissertation. How you formulate your research questions and research objectives will depend on your discipline, topic, and focus, but you should always clearly state the central aim of your research.

If your research aims to test hypotheses , you can formulate them here. Your introduction is also a good place for a conceptual framework that suggests relationships between variables .

• Conduct surveys to collect data on students’ levels of knowledge, understanding, and positive/negative perceptions of government policy.
• Determine whether attitudes to climate policy are associated with variables such as age, gender, region, and social class.
• Conduct interviews to gain qualitative insights into students’ perspectives and actions in relation to climate policy.

To help guide your reader, end your introduction with an outline  of the structure of the thesis or dissertation to follow. Share a brief summary of each chapter, clearly showing how each contributes to your central aims. However, be careful to keep this overview concise: 1-2 sentences should be enough.

I. Introduction

Human language consists of a set of vowels and consonants which are combined to form words. During the speech production process, thoughts are converted into spoken utterances to convey a message. The appropriate words and their meanings are selected in the mental lexicon (Dell & Burger, 1997). This pre-verbal message is then grammatically coded, during which a syntactic representation of the utterance is built.

Speech, language, and voice disorders affect the vocal cords, nerves, muscles, and brain structures, which result in a distorted language reception or speech production (Sataloff & Hawkshaw, 2014). The symptoms vary from adding superfluous words and taking pauses to hoarseness of the voice, depending on the type of disorder (Dodd, 2005). However, distortions of the speech may also occur as a result of a disease that seems unrelated to speech, such as multiple sclerosis or chronic obstructive pulmonary disease.

This study aims to determine which acoustic parameters are suitable for the automatic detection of exacerbations in patients suffering from chronic obstructive pulmonary disease (COPD) by investigating which aspects of speech differ between COPD patients and healthy speakers and which aspects differ between COPD patients in exacerbation and stable COPD patients.

Checklist: Introduction

I have introduced my research topic in an engaging way.

I have provided necessary context to help the reader understand my topic.

I have clearly specified the focus of my research.

I have shown the relevance and importance of the dissertation topic .

I have clearly stated the problem or question that my research addresses.

I have outlined the specific objectives of the research .

I have provided an overview of the dissertation’s structure .

You've written a strong introduction for your thesis or dissertation. Use the other checklists to continue improving your dissertation.

The introduction of a research paper includes several key elements:

• A hook to catch the reader’s interest
• Relevant background on the topic
• Details of your research problem
• A thesis statement or research question
• Sometimes an outline of the paper

Don’t feel that you have to write the introduction first. The introduction is often one of the last parts of the research paper you’ll write, along with the conclusion.

This is because it can be easier to introduce your paper once you’ve already written the body ; you may not have the clearest idea of your arguments until you’ve written them, and things can change during the writing process .

Research objectives describe what you intend your research project to accomplish.

They summarise the approach and purpose of the project and help to focus your research.

Your objectives should appear in the introduction of your research paper , at the end of your problem statement .

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George, T. & McCombes, S. (2022, September 09). How to Write a Thesis or Dissertation Introduction. Scribbr. Retrieved 22 April 2024, from https://www.scribbr.co.uk/thesis-dissertation/introduction/

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The resources in this section are designed to provide guidance for the first steps of the thesis or dissertation writing process. They offer tools to support the planning and managing of your project, including writing out your weekly schedule, outlining your goals, and organzing the various working elements of your project.

Weekly Goals Sheet (a.k.a. Life Map) [Word Doc]

This editable handout provides a place for you to fill in available time blocks on a weekly chart that will help you visualize the amount of time you have available to write. By using this chart, you will be able to work your writing goals into your schedule and put these goals into perspective with your day-to-day plans and responsibilities each week. This handout also contains a formula to help you determine the minimum number of pages you would need to write per day in order to complete your writing on time.

Setting a Production Schedule (Word Doc)

This editable handout can help you make sense of the various steps involved in the production of your thesis or dissertation and determine how long each step might take. A large part of this process involves (1) seeking out the most accurate and up-to-date information regarding specific document formatting requirements, (2) understanding research protocol limitations, (3) making note of deadlines, and (4) understanding your personal writing habits.

Creating a Roadmap (PDF)

Part of organizing your writing involves having a clear sense of how the different working parts relate to one another. Creating a roadmap for your dissertation early on can help you determine what the final document will include and how all the pieces are connected. This resource offers guidance on several approaches to creating a roadmap, including creating lists, maps, nut-shells, visuals, and different methods for outlining. It is important to remember that you can create more than one roadmap (or more than one type of roadmap) depending on how the different approaches discussed here meet your needs.

• How To Write A Thesis Introduction: Write like Professional

How To Write A Thesis Introduction: Secrets, Tips, Advice

When you write a thesis, you should pay exceptional attention to the introduction. The reader will start your thesis from the introduction, and he will make up his view and understanding of the problem, your ideas, professionalism and writing skills based on the introduction. Your introduction is an important section that must be perfect. Good news that you can read our guide on how to write a thesis introduction of high quality or find  thesis writer on our website. Keep reading and find out useful information, tips, and advice to create a successful project.

Thesis Introduction: How to Start?

A thesis introduction  is the first special chapter of your work and the starting point of your paper. If to say shortly, you should describe the topic of your thesis, talk about problem statements and write persuasive points based on an overview of your work. Let's define the main purposes of your introduction?

Main Purposes

• To introduce the topic: answer the hypothetical question of what the main reason is, what a thesis idea is and why it is relevant today?
• To win the reader’s interest. Use persuasive but clear words and facts to make your introduction interesting for the reader. People should be interested in keeping reading your dissertation.
• To demonstrate awareness of your topic as well as the relevance of the research question . The author should persuade the reader of professionalism. Your main goal is to show the entire research is relevant, and it contains significant academic and practical components.

Which Parts Should Your Introduction Contain?

The introduction should be placed in your thesis just after the table of contents. In this part, you have to provide readers with a good beginning to explain to them shortly what they will read in the document. You need to describe your research with its main purpose and direction. Below, you can find the main parts your introduction should contain:

• Context and topic. Write about what your readers need to know to understand your thesis.
• Scope and focus. Think about the certain aspect of the topic you want to address to your readers.
• Importance and relevance. Your current research must fit into work that already was done on the chosen topic.
• Objectives and questions. Define research problem , explain what's the main goal of your research and how good it gives all the needed information to readers.
• Structure overview. Each chapter of your thesis must contain something useful to explain the main goal of the writing.  

What Information Should You Give in the Introduction? 

Give only meaningful, important and accurate information in your thesis and the introduction. Thanks to the details you give here, your reader should understand:

• What is the topic of your paper;
• What part of the general topic needs further investigations, research and why;
• Your thesis statement.

Each paragraph has the number of steps you should follow, and the introduction is not an exception to this rule. Read the steps you should make when you write your introduction piece.

Writing a Thesis Introduction: Steps

These 12 steps that can be common despite the topic. However, some specific fields require more stages.

• Introduce the topic of your research problem;
• Give some general background information;
• Make a short overview of related literature you have studied (the detailed view should be written in a literature review section);
• Give the general idea of the scope of the topic you have chosen;
• Describe in details the current situation concerning the problem;
• Find the area or piece of the topic which is not researched yet or researched not enough;
• Explain the relevance, importance of the research you are going to do;
• State the main aims and objectives of your thesis;
• Name the research question (questions) or problem (problems);
• Give your hypotheses;
• Show the how to structure a thesis ;
• Outline the methodology you used.

Your introduction is the only chance to catch the interest of your reader and to make the first impression. Remember the introductory part has the same importance as all other chapters of your dissertation you have to complete.

How to Make a Well-Structured Introduction?

Above, we have mentioned the main parts that must be included in the thesis or dissertation introduction. Let's see what information you need to write in each of those parts. Make sure you read all the points thoroughly to include all the needed elements to your writing.

Context and Topic

Start with the topic introduction and provide readers with a short background. Show why the chosen subject is important to make the audience interested. It means you should select a relevant topic for discussion.

Scope and Focus

Here you have to define the scope of your dissertation and focus of your research. For example, you can mention the area of your investigation, the time period of your research, the communities you're researching, or any other specific aspects of your dissertation.

Importance and Relevance

Try to explain your motivation for this research. Provide an overview of the current state of your study and don't forget to cite sources. You will make a more detailed view of all used sources in the literature review section of your manuscript. Show the importance of your research and how it helps to solve a particular problem.  

Objectives and Questions

Pay a lot of attention to this part because here you set up the expectations of the rest of your work. Please focus on the main goal of your research to formulate your objectives and questions. Here you can provide the methods you used in your paper to answer these questions. You can write about it in detail in the methodology part. You can also include your hypotheses here. It's possible to include the hypotheses after the literature review.

Structure Overview

Your document must have a good structure to guide the reader through. Make an overview of the structure: write just 1-2 sentences to describe the content of each part of your thesis. But if the research is complicated, you can write a paragraph for each part to explain the document's structure.

How to Organize Your Work?

You don’t write your thesis in the same order as all the chapters appear in the outline. So how to organize your work effectively to complete your Ph.D. dissertation successfully?

• You should organize your whole paper in a logical consequence and write your work using the informative language;
• Find facts, figures, create infographics to illustrate your argument in details;
• Define the main parts of the thesis: Background information; Facts you will use in the argument; Observations; Ways of connecting review, analysis, and conclusions; The main elements of the thesis (sections).
• Now you can start writing.

Length of Introduction

The length is an important issue. It shouldn’t take a lot of time to read it, but it should contain all the important points (please check them above). There are no special length requirements for thesis introduction, just be clear and don’t provide unnecessary information here. At the same time, there is no need to squeeze all your points in one page. Two or three pages will be quite enough. Read our guide to find out how long is a thesis paper or its separate chapter.

Rule ‘General - Specific – General’

It is a good rule you can follow not only in thesis introductions but many other writing papers. You should start your paper with a broad position - explain why the field for your research matters and why it is relevant.

After that, you can go to narrower things. Find points which lie in your niche, share and describe details, explain why they matter to your topic.

In the end, you should think wider again. Make connections between narrow and general things to show the effects and results of your research.

How to Write an Engaging Thesis Introduction: Tips

Read the next tips if you want to know how to write a thesis introduction in a good way.

• Give the proper acknowledgment of previous research on which you are writing a thesis paper .
• In the beginning, give your readers an understanding of how you are going to structure your thesis. What will they find in chapters?
• Surprise the reader a little. Give something unexpected details: unique research or point of view, some facts or perspectives. Make your reader feel excited about reading.
• Always talk about the best experience in the field: check and present the best examples that you have, the best literature review, and the best experience of other researchers.
• Don’t use specific vocabulary in the beginning. It is like a door to your thesis for people to make it clear for understanding.
• Don’t neglect to draft. This will help you to improve your work as in the case of any other chapter.
• Use the simple present tense to state the topic/subject. Use simple past tense/present perfect tense for describing background information.
• Don’t include a lot of citations. You can provide readers with all the used sources in the literature review chapter.

It’s not easy to write a good thesis introduction. We tried to give you all the necessary information because we knew that you might have difficulties with that. But if you feel unconfident, it’s a good idea to order your thesis from professionals. If you still have any questions, you can address them to our professional writers, they will answer you with pleasure. Contact experts to finish your project easily! Our English professionals can create a perfect Ph.D. thesis to shift your expectations!

The methodology is an important part of your dissertation. It describes a broad philosophical underpinning to your chosen research methods, either quantitative or qualitative, to explain to readers your approach better. Make sure that you’re clear about an academic basis for your choice of research ...

If your college requires you to undergraduate thesis, then chances are that they want you to write a literature review. If you’re writing a literature review for academic purposes, then this document demonstrates that you have learned from the previous publications and are ready to work on new ideas...

Thesis abstract is an essential part of the dissertation paper. It is a summary of a complete work. It gives readers a chance to discover the key points of your dissertation, its research chapter, methodology and results part. Writing a proper abstract is important. Students use various techniques a...

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Home > Books > Current Trends in Wheat Research

Introductory Chapter: Current Trends in Wheat Research

Published: 11 May 2022

DOI: 10.5772/intechopen.103763

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Current Trends in Wheat Research

Edited by Mahmood-ur-Rahman Ansari

To purchase hard copies of this book, please contact the representative in India: CBS Publishers & Distributors Pvt. Ltd. www.cbspd.com | [email protected]

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Author Information

Nazia nahid.

• Department of Bioinformatics and Biotechnology, GC University–Faisalabad, Pakistan

Parwsha Zaib

Tayyaba shaheen *, kanval shaukat.

• Department of Botany, University of Balochistan, Pakistan

Akmaral U. Issayeva

• M. Auezov South Kazakhstan University, Kazakhstan

Mahmood-ur-Rahman Ansari *

*Address all correspondence to: [email protected] and [email protected]

1. Introduction

Wheat ( Triticum aestivum ) is known as one of the most important cereal crops and is extensively grown worldwide [ 1 ]. Wheat contributes to 50% and 30% of the global grain trade and production respectively [ 2 ]. Wheat is also known as a staple food in more than 40 countries of the world. Wheat provides 82% of basic calories and 85% of proteins to the world population [ 3 , 4 ]. Wheat-based food is rich in fiber contents than meat-based food. Dough produced from bread wheat flour has different viscoelastic properties than other cereals. It is considered a higher fiber food. Therefore, its positive effects on controlling cholesterol, glucose, and intestinal functions in the body were observed [ 5 ]. Primarily, wheat is being used to make Chapatti (Bread) but it also contributes to other bakery products. Wheat utility and high nutritional value made it the staple food for more than 1/3rd population of the world. Wheat grain is separated from the chaff and stalks after the harvesting of wheat. Stalks of wheat are further used in animal bedding and construction material. Globally, the need for wheat production is enhancing even in countries having unfavorable climates for its production. Global climate changes are badly affecting the production of wheat and it raised the concern for food security.

It is estimated that annual cereal production should be increased by 1 billion tons to feed the expected population of 9.1 billion by 2050 [ 1 ]. The current scenario demands an increase in crop productivity to meet the increased requirements of food supply [ 6 ]. Wheat is grown in tropical and subtropical regions which experiences a lot of stress. These stresses result in a reduction of yield [ 7 ]. Major environmental stresses include cold, salinity, heat, and drought which are drastically affecting its yield. However, water and heat are considered as the key environmental stresses which caused in reduction of the wheat yield globally [ 8 , 9 ]. So, genetic improvements related to yield and stress tolerance are mandatory to enhance the production of wheat [ 10 , 11 ].

2. Genetically modified wheat plants

Genetically modified wheat plants have been produced by the use of bacteria. Wheat plants were inoculated with the plant-growth-promoting bacteria (PGPB) which resulted in the higher expression of abiotic stress (mainly drought and salinity) tolerant genes [ 12 ]. PGPB inoculated wheat cultivars also showed the higher expression of genes encoding antioxidant-enzymes, such as catalase (CAT), peroxidase , ascorbate peroxidase (APX), and glutathione peroxidase (GPX). So, it was concluded that PGPB used in wheat plants resulted in increased tolerance to abiotic stresses [ 12 ]. Cold shock proteins increase the survival of bacteria in severe environmental conditions. CspA and CspB genes from bacteria were transformed into wheat. Transgenic wheat plants expressing SeCspA and SeCspB were observed to have decreased water loss rate, increased proline and chlorophyll contents under salinity, and less water-stress conditions [ 13 ]. It was further investigated that SeCsp transgenic wheat plants resulted in enhanced weight and yield of grain than the control plants. SeCspA transgenic wheat plants were observed to have an improved water-stress tolerance than the control plants ( Table 1 , [ 13 ]).

Development of transgenic wheat having various traits/phenotypes.

Gluten is a protein comprised of gliadins found in wheat. Gluten is the main cause of coeliac disease in individuals. Bread-making quality of wheat is determined by the gluten proteins. Wheat varieties with less gliadin contents were produced using gene-editing technologies and RNAi (RNA interference). Wheat lines lacking immunogenic gluten were produced. Low immunogenic gluten and more nutritional values were added in one wheat line named E82. A better microbiota profile (protection microorganisms available in the gut) was observed in the NCWS patients using the bread made with E82 [ 28 ]. Plant cuticle has a positive role in the protection of plant against biotic and abiotic stresses. Wheat plants transformed with TaSHN1 resulted in increased water-stress tolerance by reducing the leaf stomatal density and changing the composition of the cuticle [ 29 ].

3. Biotic stress tolerance in wheat

Wheat is considered an excessive contributor toward the human calorie intake [ 30 ]. Pests and pathogens cause yield losses in wheat up to 21.5% of the total losses and could be reached to 28.1% [ 31 ]. Wheat is affected by the fungal disease, powdery mildew caused by Blumeria graminis f. sp. tritici (Bgt). Powdery mildew is a damaging disease that resulted in greater loss of wheat [ 32 ]. Broad-spectrum resistant genes (BSR) are considered to have the most significant role to control powdery mildew. CMPG1-V gene was cloned from the Hynaldia villosa and it was observed that higher expression of CMPG1-V gene resulted in the Broad-spectrum resistance against powdery mildew [ 33 , 34 ]. Barley chi26 gene could also be used to enhance the resistance against powdery mildew and rust through genetic modification [ 35 ]. Some epigenetic regulators were determined to have a role in wheat powdery mildew resistance. TaHDT701 is a histone deacetylase that was found as a negative regulator of wheat defense against powdery mildew. TaHDT701 was observed to be associated with the one repeat protein (TaHOS15) and RPD3 type histone deacetylase TaHDA6. Knockdown of this histone deacetylase complex ( TaHDT701 , TaHDA6 , TaHOS15 ) in wheat resulted in increased powdery mildew tolerance [ 36 ].

Fusarium graminearum is a plant fungal pathogen that causes a devastating disease called Fusarium head blight in wheat. It results in the reduction of wheat production. Genetic techniques were used to increase the FHB (Fusarium head blight) resistance in wheat. Transgenic wheat plants expressing barley class II chitinase gene 2 were observed to have a higher resistance against Fusarium graminearum [ 37 ]. Lr10 and Lr21 were cloned and transformed into wheat. The transgenic plants were reported to be resistant to leaf rust disease. Evolution and diversification of HIPPs (heavy metal-associated isoprenylated plant proteins) genes were studied in Triticeae [ 38 ]. HIPPs genes of Hynaldia villosa were cloned through homology-based cloning. Transgenic wheat having HIPP1-V was developed and the role of HIPP1-V in cadmium stress was characterized. It was observed that higher expression of this gene resulted in increased tolerance to cadmium stress. Therefore, HIPP1-V could be used to increase the tolerance in wheat against cadmium [ 39 ].

4. Abiotic stress tolerance in wheat

Grain number, weight, and size are greatly reduced under the negative effects of environmental stresses. However, the timing, duration, and intensity of stress determine the severity of the negative effects [ 40 , 41 ]. Wheat is a major source of protein and calories for the human diet. High temperature is badly affecting the yield of wheat which is a main concern worldwide. Drought and heat stresses are the two main abiotic stresses which are playing a greater role in the reduction of wheat yield. Reduction in starch contents, photosynthetic activity, grain number, and chlorophyll contents in the endosperm is caused due to rise in temperature. Heat stress results in the accumulation of reactive oxygen species (ROS) which is the main reason for higher oxidative damage to the plant. Heat stress also results in the variation of wheat biochemistry, morphology, and physiology. Tolerance, avoidance, and escape are known as the three major mechanisms that support the plant to grow in a heat-stress environment. Major heat tolerance mechanisms in wheat are known as stay green, heat shock proteins, and antioxidant defense [ 42 ]. Protein synthesis and folding were observed to be interrupted during heat stress. Heat stress also resulted in the production of several stress agents badly affecting transcription, translation, and DNA replication in plants [ 43 ]. Plants speed up the production of heat shock proteins as a defense mechanism [ 44 ]. Higher activity of antioxidants, such as peroxidases, catalase, and superoxide dismutase, was observed under heat stress. Wheat cultivar showing greater tolerance to heat stress was observed to have higher activity of catalase, ascorbate peroxidase, and S-transferase [ 45 ].

Salt stress greatly affects the growth of wheat plants. Salinity stress has a higher impact on the morphology and physiology of wheat plants. Plants having less tolerance to salinity are not suitable for cropping. Potassium transporter ( HKT ) genes have a greater role in achieving salinity tolerance in wheat. Sodium (Na + ) exclusion through HKT genes is a major mechanism in wheat to have a salinity tolerance. OsMYBSs and AtAB14 are the transcription factors having a role in regulating HKT genes, which are considered as the candidate targets for increasing salinity tolerance in wheat [ 46 ]. Wheat transformed with a mutated transcription factor, HaHB4 showed higher water-use efficiency and was more yielding under drought stress [ 26 ]. Transgenic wheat expressing GmDREB1 gene from soybean was also observed to have higher drought tolerance under water-stress conditions [ 47 ]. DREB1A gene from Arabidopsis thaliana was introduced to bread wheat and increased tolerance against water stress in the transgenic wheat was observed. Bread wheat under drought stress was observed to have a higher level of WRKY proteins [ 48 ]. Higher expression of AtHDG11 gene in transgenic wheat resulted in increased water-stress tolerance during drought-stress conditions. Enhanced TaNAC69 expression in root and leaf of wheat during drought stress was observed [ 49 ]. Researchers are working to develop transgenic wheat having various traits/phenotypes by using advanced approaches of biotechnology for the last several decades ( Table 1 ). Numbers of transgenic wheat cultivars are being grown in the fields and several more are under trial.

5. CRISPR/Cas9 system in wheat

Gliadins and glutenins are known as the gluten proteins and ingestion of these proteins from barley, rye, and wheat could cause the disease called coeliac disease in humans. The only remedy is to develop gluten-free food. Transgenic wheat which retains baking quality and is safe for coeliac could not be produced using conventional methods because of the complexity of the wheat genome. Coeliac disease (CD) is activated by the immunogenic isotopes mainly gliadins. Gliadin families were downregulated by the use of RNA interference. CRISPR/Cas9 is a targeted gene manipulation tool considered to have a potential role in genetic modification ( Table 2 , [ 60 , 61 ]). CRISPR/Cas9 system was recently used for gene editing of gliadins. Offsprings with deleted, edited, or silenced gliadins were produced by CRISPR/Cas9. They helped to decrease the exposure of the patient to the CD epitopes [ 62 ]. This technology has been used to develop wheat cultivars having gluten genes with inactivated CD epitopes [ 62 , 63 ].

Genome edited wheat developed by CRISPR/Cas9 system.

CRISPR/Cas9 system and TALENS (transcription activator-like effector nuclease) were used in the bread wheat to generate the mutations in three homoeoalleles that encode MLO locus proteins against mildew. Mutations in all three TaMLO were generated by using TALENS which resulted in resistance against powdery mildew. The MLO homoeoalleles ( TaMLOA1 , TaMLOB1, and TaMLOD1 ) of bread wheat contributed to the mildew infection. Mutation of MLO alleles resulted in powdery mildew tolerance in wheat [ 50 ]. Genome editing was reported in which pds (phytoene desaturase) and inox (inositol oxygenase) genes in the cell suspension-culture of wheat were targeted. It was demonstrated that the genome-editing technique could also be applied in the cell suspension of wheat [ 64 ]. Very recently, various research groups are involved to develop transgenic wheat by using genome-editing technology. Some of the experiments are listed in Table 2 .

6. Wheat computational analysis

A comprehensive resource for wheat reference genome was developed by International Wheat Genome Sequencing Consortium. The URGI portal ( https://wheat-urgi.versailles.inra.fr/ ) was developed for the breeders and researchers to access the genome sequence data of bread-wheat. InterMine tools, genome browser, and BLAST were established for the exploration of genome sequences together with the additional linked datasets, including gene expression, physical maps, and sequence variation. Portal provided the higher browser and search features that facilitated the use of the latest genomic resources required for the upgradation of wheat [ 65 ].

DNA binding with one finger (Dof) transcription factors is known to have an important role in abiotic stress tolerance as well as the growth of plants. Ninety-six TaDof members of the gene family have been studied using computational approaches. By qPCR analysis, it was revealed that TaDof genes were upregulated under heavy metal and heat stress in wheat. Consequently, it could be concluded that detection of amino acid sites, genome-wide analysis, and identification of the Dof transcription factor family could provide us the new insight into the function, structure, and evolution of the Dof gene family [ 66 ].

Acknowledgments

This work was supported by funds from the Higher Education Commission of Pakistan.

• 1. FAO. World Food and Agriculture-FAO Statistical Pocketbook. Rome, Italy: FAO; 2015
• 2. Akter N, Islam MR. Heat stress effects and management in wheat: A review. Agronomy for Sustainable Development. 2017; 37 (5):1-17
• 3. Chaves MS, Martinelli JA, Wesp-Guterres C, Graichen FAS, Brammer SP, Scagliusi SM, et al. The importance for food security of maintaining rust resistance in wheat. Food Security. 2013; 5 (2):157-176
• 4. Sharma D, Singh R, Tiwari R, Kumar R, Gupta VK. Wheat responses and tolerance to terminal heat stress: A review. In: Hasanuzzaman M, Nahar K, Hossain M, editors. Wheat Production in Changing Environments. Singapore: Springer; 2019. pp. 149-173
• 5. Giraldo P, Benavente E, Manzano-Agugliaro F, Gimenez E. Worldwide research trends on wheat and barley: A bibliometric comparative analysis. Agronomie. 2019; 9 (7):352
• 6. Iqbal M, Raja NI, Yasmeen F, Hussain M, Ejaz M, Shah MA. Impacts of heat stress on wheat: A critical review. Advances in Crop Science and Technology. 2017; 5 (1):01-09
• 7. Rahaie M, Xue GP, Schenk PM. The role of transcription factors in wheat under different abiotic stresses. Abiotic Stress - Plant Responses and Applications in Agriculture. 2013; 2 :367-385
• 8. Lesk C, Rowhani P, Ramankutty N. Influence of extreme weather disasters on global crop production. Nature. 2016; 529 (7584):84-87
• 9. Liu B, Asseng S, Müller C, Ewert F, Elliott J, Lobell DB, et al. Similar estimates of temperature impacts on global wheat yield by three independent methods. Nature Climate Change. 2016; 6 (12):1130-1136
• 10. Tester M, Langridge P. Breeding technologies to increase crop production in a changing world. Science. 2010; 327 (5967):818-822
• 11. He Z, Joshi AK, Zhang W. Climate vulnerabilities and wheat production. In: Pielke RA, editor. Climate Vulnerability: Understanding and Addressing Threats to Essential Resources. Waltham: Academic Press; 2013. pp. 57-67
• 12. Sudan J, Sharma D, Mustafiz A, Kumari S. Signaling peptides: Hidden molecular messengers of abiotic stress perception and response in plants. In: Zargar S, Zargar M, editors. Abiotic Stress-Mediated Sensing and Signaling in Plants: An Omics Perspective. Singapore: Springer; 2018. pp. 95-125
• 13. Yu TF, Xu ZS, Guo JK, Wang YX, Abernathy B, Fu JD, et al. Improved drought tolerance in wheat plants overexpressing a synthetic bacterial cold shock protein gene SeCspA. Scientific Reports. 2017; 7 (1):1-4
• 14. Peña PA, Quach T, Sato S, Ge Z, Nersesian N, Changa T, et al. Expression of the maize Dof1 transcription factor in wheat and sorghum. Frontiers in Plant Science. 2017; 8 :434
• 15. Qu B, He X, Wang J, Zhao Y, Teng W, Shao A, et al. A wheat CCAAT box-binding transcription factor increases the grain yield of wheat with less fertilizer input. Plant Physiology. 2015; 167 (2):411-423
• 16. Yadav D, Shavrukov Y, Bazanova N, Chirkova L, Borisjuk N, Kovalchuk N, et al. Constitutive overexpression of the TaNF-YB4 gene in transgenic wheat significantly improves grain yield. Journal of Experimental Botany. 2015; 66 (21):6635-6650
• 17. He X, Qu B, Li W, Zhao X, Teng W, Ma W, et al. The nitrate-inducible NAC transcription factor TaNAC2-5A controls nitrate response and increases wheat yield. Plant Physiology. 2015; 169 (3):1991-2005
• 18. Tian B, Talukder SK, Fu J, Fritz AK, Trick HN. Expression of a rice soluble starch synthase gene in transgenic wheat improves the grain yield under heat stress conditions. In Vitro Cellular & Developmental Biology. Plant. 2018; 54 (3):216-227
• 19. Hu M, Zhao X, Liu Q , Hong X, Zhang W, Zhang Y, et al. Transgenic expression of plastidic glutamine synthetase increases nitrogen uptake and yield in wheat. Plant Biotechnology Journal. 2018; 16 (11):1858-1867
• 20. Smidansky ED, Meyer FD, Blakeslee B, Weglarz TE, Greene TW, Giroux MJ. Expression of a modified ADP-glucose pyrophosphorylase large subunit in wheat seeds stimulates photosynthesis and carbon metabolism. Planta. 2007; 225 (4):965-976
• 21. Zhao XQ , Nie XL, Xiao XG. Over-expression of a tobacco nitrate reductase gene in wheat (Triticum aestivum L.) increases seed protein content and weight without augmenting nitrogen supplying. PLoS One. 2013; 8 (9):e74678
• 22. Connorton JM, Jones ER, Rodríguez-Ramiro I, Fairweather-Tait S, Uauy C, Balk J. Wheat vacuolar iron transporter TaVIT2 transports Fe and Mn and is effective for biofortification. Plant Physiology. 2017; 174 (4):2434-2444
• 23. Yang Y, Luang S, Harris J, Riboni M, Li Y, Bazanova N, et al. Overexpression of the class I homeodomain transcription factor Ta HDZ ipI-5 increases drought and frost tolerance in transgenic wheat. Plant Biotechnology Journal. 2018; 16 (6):1227-1240
• 24. Saint Pierre C, Crossa JL, Bonnett D, Yamaguchi-Shinozaki K, Reynolds MP. Phenotyping transgenic wheat for drought resistance. Journal of Experimental Botany. 2012; 63 (5):1799-1808
• 25. Castiglioni P, Warner D, Bensen RJ, Anstrom DC, Harrison J, Stoecker M, et al. Bacterial RNA chaperones confer abiotic stress tolerance in plants and improved grain yield in maize under water-limited conditions. Plant Physiology. 2008; 147 (2):446-455
• 26. González FG, Capella M, Ribichich KF, Curín F, Giacomelli JI, Ayala F, et al. Field-grown transgenic wheat expressing the sunflower gene HaHB4 significantly outyields the wild type. Journal of Experimental Botany. 2019; 70 (5):1669-1681
• 27. Shavrukov Y, Baho M, Lopato S, Langridge P. The TaDREB3 transgene transferred by conventional crossings to different genetic backgrounds of bread wheat improves drought tolerance. Plant Biotechnology Journal. 2016; 14 :313-322
• 28. García-Molina MD, Giménez MJ, Sánchez-León S, Barro F. Gluten free wheat: Are we there? Nutrients. 2019; 11 (3):487
• 29. Bi H, Shi J, Kovalchuk N, Luang S, Bazanova N, Chirkova L, et al. Overexpression of the TaSHN1 transcription factor in bread wheat leads to leaf surface modifications, improved drought tolerance, and no yield penalty under controlled growth conditions. Plant, Cell and Environment. 2018; 41 (11):2549-2566
• 30. Godfray HC, Beddington JR, Crute IR, Haddad L, Lawrence D, Muir JF, et al. Food security: The challenge of feeding 9 billion people. Science. 2010; 327 (5967):812-818
• 31. Savary S, Willocquet L, Pethybridge SJ, Esker P, McRoberts N, Nelson A. The global burden of pathogens and pests on major food crops. Nature Ecology & Evolution. 2019; 3 (3):430-439
• 32. Menardo F, Praz CR, Wyder S, Ben-David R, Bourras S, Matsumae H, et al. Hybridization of powdery mildew strains gives rise to pathogens on novel agricultural crop species. Nature Genetics. 2016; 48 (2):201-205
• 33. Zhu Y, Li Y, Fei F, Wang Z, Wang W, Cao A, et al. E3 ubiquitin ligase gene CMPG 1–V from Haynaldia villosa L. contributes to powdery mildew resistance in common wheat (Triticum aestivum L.). The Plant Journal. 2015; 84 (1):154-168
• 34. Liu J, Sun L, Chen Y, Wei L, Hao Y, Yu Z, et al. The regulatory network of CMPG1-V in wheat–Blumeria graminis f. sp. tritici interaction revealed by temporal profiling using RNA-Seq. International Journal of Molecular Sciences. 2020; 21 (17):5967
• 35. Eissa HF, Hassanien SE, Ramadan AM, El-Shamy MM, Saleh OM, Shokry AM, et al. Developing transgenic wheat to encounter rusts and powdery mildew by overexpressing barley chi26 gene for fungal resistance. Plant Methods. 2017; 13 (1):1-3
• 36. Zhi P, Kong L, Liu J, Zhang X, Wang X, Li H, et al. Histone deacetylase TaHDT701 functions in TaHDA6-TaHOS15 complex to regulate wheat defense responses to Blumeria graminis f. sp. tritici. International Journal of Molecular Sciences. 2020; 21 (7):2640
• 37. Shin S, Mackintosh CA, Lewis J, Heinen SJ, Radmer L, Dill-Macky R, et al. Transgenic wheat expressing a barley class II chitinase gene has enhanced resistance against Fusarium graminearum. Journal of Experimental Botany. 2008; 59 (9):2371-2378
• 38. Zhang H, Zhang X, Liu J, Niu Y, Chen Y, Hao Y, et al. Characterization of the heavy-metal-associated Isoprenylated plant protein (HIPP) gene family from Triticeae species. International Journal of Molecular Sciences. 2020; 21 (17):6191
• 39. Hura T. Wheat and barley: Acclimatization to abiotic and biotic stress. International Journal of Molecular Sciences. 2020; 21 (19):7423
• 40. Foulkes MJ, Scott RK, Sylvester-Bradley R. The ability of wheat cultivars to withstand drought in UK conditions: Formation of grain yield. The Journal of Agricultural Science. 2002; 138 (2):153-169
• 41. Weldearegay DF, Yan F, Jiang D, Liu F. Independent and combined effects of soil warming and drought stress during anthesis on seed set and grain yield in two spring wheat varieties. Journal of Agronomy and Crop Science. 2012; 198 (4):245-253
• 42. Poudel PB, Poudel MR. Heat stress effects and tolerance in wheat: A review. Journal of Biology and Today's World. 2020; 9 (3):1-6
• 43. Biamonti G, Caceres JF. Cellular stress and RNA splicing. Trends in Biochemical Sciences. 2009; 34 (3):146-153
• 44. Gupta SC, Sharma A, Mishra M, Mishra RK, Chowdhuri DK. Heat shock proteins in toxicology: How close and how far? Life Sciences. 2010; 86 (11-12):377-384
• 45. Balla K, Bencze S, Janda T, Veisz O. Analysis of heat stress tolerance in winter wheat. Acta Agronomica Hungarica. 2009; 57 (4):437-444
• 46. Kunika BK, Singh PK, Rani V, Pandey GC. Salinity tolerance in wheat: An overview. International Journal of Chemical Studies. 2019; 6 :815-820
• 47. Shiqing GA, Huijun XU, Xianguo C, Ming C, Zhaoshi XU, Liancheng L, et al. Improvement of wheat drought and salt tolerance by expression of a stress-inducible transcription factor GmDREB of soybean (Glycine max). Chinese Science Bulletin. 2005; 50 :2714-2723, 2723
• 48. Okay S, Derelli E, Unver T. Transcriptome-wide identification of bread wheat WRKY transcription factors in response to drought stress. Molecular Genetics and Genomics. 2014; 289 (5):765-781
• 49. Xue GP, Bower NI, McIntyre CL, Riding GA, Kazan K, Shorter R. TaNAC69 from the NAC superfamily of transcription factors is up-regulated by abiotic stresses in wheat and recognises two consensus DNA-binding sequences. Functional Plant Biology. 2006; 33 (1):43-57
• 50. Wang Y, Cheng X, Shan Q , Zhang Y, Liu J, Gao C, et al. Simultaneous editing of three homoeoalleles in hexaploid bread wheat confers heritable resistance to powdery mildew. Nature Biotechnology. 2014; 32 (9):947-951
• 51. Ouyang X, Hong X, Zhao X, Zhang W, He X, Ma W, et al. Knock out of the PHOSPHATE 2 gene TaPHO2-A1 improves phosphorus uptake and grain yield under low phosphorus conditions in common wheat. Scientific Reports. 2016; 6 :29850
• 52. Zhang Y, Liang Z, Zong Y, Wang Y, Liu J, Chen K, et al. Efficient and transgene-free genome editing in wheat through transient expression of CRISPR/Cas9 DNA or RNA. Nature Communications. 2016; 7 :12617
• 53. Zhang Y, Bai Y, Wu G, Zou S, Chen Y, Gao C, et al. Simultaneous modification of three homoeologs of TaEDR1 by genome editing enhances powdery mildew resistance in wheat. The Plant Journal. 2017; 91 :714-724
• 54. Wang W, Mauleon R, Hu Z, Chebotarov D, Tai S, Wu Z, et al. Genomic variation in 3,010 diverse accessions of Asian cultivated rice. Nature. 2018; 557 (7703):43-49
• 55. Okada A, Arndell T, Borisjuk N, Sharma N, Watson-Haigh NS, Tucker EJ, et al. CRISPR/Cas9-mediated knockout of Ms1 enables the rapid generation of male-sterile hexaploid wheat lines for use in hybrid seed production. Plant Biotechnology Journal. 2019; 17 :1905-1913
• 56. Li J, Jiao G, Sun Y, Chen J, Zhong Y, Yan L, et al. Modification of starch composition, structure and properties through editing of TaSBEIIa in both winter and spring wheat varieties by CRISPR/Cas9. Plant Biotechnology Journal. 2020; 19 :937-951
• 57. Zong Y, Wang Y, Li C, Zhang R, Chen K, Ran Y, et al. Precise base editing in rice, wheat and maize with a Cas9-cytidine deaminase fusion. Nature Biotechnology. 2017; 35 :438-440
• 58. Zhang R, Liu J, Chai Z, Chen S, Bai Y, Zong Y, et al. Generation of herbicide tolerance traits and a new selectable marker in wheat using base editing. Nature Plants. 2019; 5 :480-485
• 59. Zhang Y, Malzahn AA, Sretenovic S, Qi Y. The emerging and uncultivated potential of CRISPR technology in plant science. Nature Plants. 2019; 5 :778-794
• 60. Schaart JG, van de Wiel CC, Lotz LA, Smulders MJ. Opportunities for products of new plant breeding techniques. Trends in Plant Science. 2016; 21 (5):438-449
• 61. Van de Wiel CC, Schaart JG, Lotz LA, Smulders MJ. New traits in crops produced by genome editing techniques based on deletions. Plant Biotechnology Reports. 2017; 11 (1):1-8
• 62. Jouanin A, Gilissen LJ, Schaart JG, Leigh FJ, Cockram J, Wallington EJ, et al. CRISPR/Cas9 gene editing of gluten in wheat to reduce gluten content and exposure—Reviewing methods to screen for coeliac safety. Frontiers in Nutrition. 2020; 7 :51
• 63. Jouanin A, Schaart JG, Boyd LA, Cockram J, Leigh FJ, Bates R, et al. Outlook for coeliac disease patients: Towards bread wheat with hypoimmunogenic gluten by gene editing of α-and γ-gliadin gene families. BMC Plant Biology. 2019; 19 (1):1-6
• 64. Upadhyay SK, Kumar J, Alok A, Tuli R. RNA-guided genome editing for target gene mutations in wheat. G3: Genes, Genomes, Genetics. 2013; 3 (12):2233-2238
• 65. Alaux M, Rogers J, Letellier T, Flores R, Alfama F, Pommier C, et al. Linking the international wheat genome sequencing consortium bread wheat reference genome sequence to wheat genetic and phenomic data. Genome Biology. 2018; 19 (1):1
• 66. Liu Y, Liu N, Deng X, Liu D, Li M, Cui D, et al. Genome-wide analysis of wheat DNA-binding with one finger (Dof) transcription factor genes: Evolutionary characteristics and diverse abiotic stress responses. BMC Genomics. 2020; 21 (1):1-8

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DISCOVERY OF NOVEL DISEASE BIOMARKERS AND THERAPEUTICS USING MACHINE LEARNING MODELS

In the fields of computational biology and bioinformatics, the identification of novel disease biomarkers and therapeutic strategies, especially for cancer diseases, remains a crucial challenge. The advancement in computer science, particularly machine learning techniques, has greatly empowered the study of computational biology and bioinformatics for their unprecedented prediction power. This thesis explores how to utilize classic and advanced machine learning models to predict prognostic pathways, biomarkers, and therapeutics associated with cancers.

Firstly, this thesis presents a comprehensive overview of computational biology and bioinformatics, covering past milestones to current groundbreaking advancements, providing context for the research. A centerpiece of this thesis is the introduction of the Pathway Ensemble Tool and Benchmark, an original methodology designated for the unbiased discovery of cancer-related pathways and biomarkers. This toolset not only enhances the identification of crucial prognostic components distinguishing clinical outcomes in cancer patients but also guides the development of targeted drug treatments based on these signatures. Inspired by Benchmark, we extended the methodology to single-cell technologies and proposed scBenchmark and PathPCA, which provides insights into the potential and limitations of how novel techniques can benefit biomarker and therapeutic discovery. Next, the research progresses to the development of DREx, a deep learning model trained on large-scale transcriptome data, for predicting gene expression responses to drug treatments across multiple cell lines. DREx highlights the potential of advanced machine learning models in drug repurposing using a genomics-centric approach, which could significantly enhance the efficiency of initial drug selection.

The thesis concludes by summarizing these findings and highlighting their importance in advancing cancer-related biomarkers and drug discovery. Various computational predictions in this work have already been experimentally validated, showcasing the real-life impact of these methodologies. By integrating machine learning models with computational biology and bioinformatics, this research pioneers new standards for novel biomarker and therapeutics discovery.

Degree Type

• Doctor of Philosophy
• Computer Science

Campus location

• West Lafayette

Advisor/Supervisor/Committee Chair

Additional committee member 2, additional committee member 3, additional committee member 4, usage metrics.

• Other information and computing sciences not elsewhere classified

Product Design Thesis Showcase

Posted in: Announcements

We are thrilled to extend to you a special invitation to Montclair State University’s highly anticipated Product Design Thesis Showcase! This event promises to be an inspiring celebration of creativity, innovation, and the culmination of months of hard work by our talented product design students. Whether you’re an industry professional seeking fresh talent, a design enthusiast looking for inspiration, or simply curious about the future of product design, this event is sure to captivate and inspire you featuring groundbreaking concepts, captivating presentations, and an engaging interactive Q&A session.

For nearly two decades, our renowned Product Design program has been dedicated to shaping the next generation of industry leaders. We take immense pride in our graduates’ achievements, with many securing coveted positions at over 80 major companies across the US and abroad, including prestigious names like Movado Group, Under Armour, Nike, and more.

Our program’s success is rooted in a robust curriculum that emphasizes Design Thinking and User-centered Design. We’ve forged valuable partnerships with leading industry organizations such as IDSA and esteemed companies like Revo, Helen of Troy, and Movado Group, offering our students unparalleled opportunities to engage with professionals in real-world settings. However, our students’ accomplishments extend far beyond the classroom. They’ve demonstrated their prowess by clinching top honors in national competitions, including 1st, 2nd, and 3rd place in the National Traffic Safety Design Competition and 2nd place in the Under Armour design competition. Moreover, their commitment to community projects is commendable, having contributed their talents to initiatives like designing habitat rooms for the Montclair Animal Shelter and building a playground for the Montclair YMCA.

We cordially invite you to witness firsthand the remarkable talent and dedication of our senior Product Design students. Your presence will undoubtedly enhance this celebration of creativity and innovation.

• Product Design Senior Presentation
• May 6, 2024, 6:00 pm
• School of Communication and Media Presentation Hall